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. Author manuscript; available in PMC: 2023 Jul 21.
Published in final edited form as: Cell Chem Biol. 2022 Jun 15;29(7):1162–1173.e5. doi: 10.1016/j.chembiol.2022.05.008

Figure 6. HDAC7-NCoR dissociation in the presence of ER.

Figure 6.

HDAC7 wild type (WT) or GOF mutant were co-expressed with wild type ER in HEK293 cells, followed by lysis, immunoprecipitation (IP) of HDAC-Flag from the lysates, and western blot analysis with NCoR, Flag (HD7-F), ER antibodies. Repetitive independent trials are shown in Figure S9. All trials include a bead binding control using lysates without expression of HDAC-Flag (IgG).