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Northern analysis of the alpha-toxin message in RNA from post-exponential-phase cultures of S. aureus strains (A) PM466, (B) PM614, and (C) PM720. The leftmost lane contains 30 μg of total RNA serially diluted (1:2) in subsequent lanes. The transcript was identified by hybridization using digoxigenin-labeled probe specific for the gene encoding alpha-toxin with chemiluminescent chemistry.
