Figure 5.

Results from IP-mass spectrometric analysis of proteins co-immunoprecipitating with HA-VMA21 WT or MUT. (A) The cryo-EM structure of human V-ATPase (ProteinDataBankID 6WM2,Mol* [36] and RCSB PDB) as published by Wang et al. in 2020 [26]. (B) Heatmap display of V-ATPase proteins co-immunoprecipitating with HA-VMA21 MUT or WT proteins based on n = 2 independent experiments. The data in B-D are normalized incorporating the normalized spectral absorbance factor (NSAF) and the relative amounts of immunoprecipitated VMA21 WT or MUT proteins. Displayed is the log₂ of MUT:WT protein amounts. (C) Heatmap display of COPI complex proteins co-immunoprecipitating with HA-VMA21 MUT or WT proteins. (D) Heatmap display of DDOST (dolichyl-diphosphooligosaccharide—protein glycosyltransferase non-catalytic subunit) complex proteins co-immunoprecipitating with HA-VMA21 MUT or WT proteins.