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. 2021 Dec 7;18(8):1841–1863. doi: 10.1080/15548627.2021.2002109

Figure 3.

Figure 3.

PLA2G4E might induce LMP in the ischemic flap. (A) Pathway analysis showing the top 20 pathways involved in differences between area I and area II. (B) PLA2G4E and p-PLA2G4E protein levels in the skin from both areas on postoperative day 7. (C) Quantification of the phosphorylation level of PLA2G4E in the skin. Data are presented as the means ± SEM (n = 6). (D) Immunofluorescence staining of LAMP1 and p-PLA2G4E in the skin from both areas on postoperative day 7. Scale bars: 10 μm. (E) Comparison of the number of p-PLA2G4E-positive lysosomes in each cell of the dermal layer between area I and area II. Data are expressed as the means ± SEM (n = 6). (F) ELISA results showing the activity of PLA2G4E in the skin from both areas on postoperative day 7. Data are expressed as the means ± SEM (n = 6). (G-J) ELISA results of the activity of lysosomal enzymes (H and J) CTSD and (G and I) NAGLU in lysosomal and cytosolic fractions extracted from the skin of both areas on postoperative day 7. Data are expressed as means ± SEM (n = 6 per group). Significance: *p < 0.05.