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. 2021 Dec 7;18(8):1841–1863. doi: 10.1080/15548627.2021.2002109

Figure 9.

Figure 9.

Overexpression of Mir504-5p suppressed PLA2G4E, stabilized the lysosomal membrane and inhibited necroptosis in vivo. (A) Western blotting to detect the PLA2G4E expression levels in the skin from area II in the saline, AAV-scramble-II and AAV-Mir504-5p-up groups on postoperative day 7. (B) Quantification of the optical density values of PLA2G4E in each group. Data are expressed as the mean ± SEM (n = 6). (C) Immunofluorescence staining of LAMP1 and p-PLA2G4E in the skin from area II in each group on postoperative day 7. Scale bars: 10 μm. (D) Comparison of the number of p-PLA2G4E-positive lysosomes in each cell of the dermal layer among the three groups. Data are expressed as the means ± SEM (n = 6). (E) ELISA results showing the activity of PLA2G4E in the skin from area II in each group on postoperative day 7. Data are expressed as the means ± SEM (n = 6). (F) Immunofluorescence staining of CTSD in the skin from area II in each group on postoperative day 7. Scale bars: 10 μm. (G) Comparison of the ratio of diffuse CTSD cells in the dermal layer among the three groups. Data are expressed as the means ± SEM (n = 6). (H) Immunofluorescence staining of p-MLKL in the skin from area II in each group on postoperative day 7. Scale bars: 10 μm. (I) Quantification of the integrated intensity of p-MLKL in the dermal layer. Data are expressed as the means ± SEM (n = 6). (J) Immunofluorescence staining of SQSTM1 in the skin from area II in each group on postoperative day 7. Scale bars: 10 μm. (K) Quantification of the integrated intensity of SQSTM1 in the dermal layer. Data are expressed as the means ± SEM (n = 6). Significance: *p < 0.05.