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. 2021 Dec 6;18(8):1822–1840. doi: 10.1080/15548627.2021.2002108

Figure 4.

Figure 4.

SH3BGRL renders autophagy of breast cancers. (A) Immunoblots of SQSTM1 protein in the MCF-7 cells with SH3BGRL overexpression or MDA-MB-453 cells with SH3BGRL knockdown, respectively. Cells were treated with or without 0.5 µg/ml actinomycin D (Act D), 50 μg/ml cycloheximide (CHX), 20 μM MG132, or 50 μM CQ for 12 h, respectively. (B) Immunoblots of MTOR and its phosphorylated form in the indicated cells. (C,D). Representative IHC staining of total LC3B protein expression in 25 paired human breast cancer tissues compared with adjacent normal ones (C) Scale bars: 100 mm (insets, 50 mm). Statistical LC3B expression is shown (D), P = 0.0109. (E) Correlation analysis of SH3BGRL expression level with that of LC3B in breast cancer tissues. (n = 25; r = −0.56; P = 0.0034).