Table 2. Difference of non-target OTU number (average per sample) and chlorophyll-a concentration (average per sample) between MiFish-0/qPCR-1 and MiFish-1/qPCR-1 samples.
| Non-target OTU number | Chlorophyll-a concentration (mg/m3) | ||||||
|---|---|---|---|---|---|---|---|
| chub/blue mackerel | anchovy | sardine | chub/blue mackerel | anchovy | sardine | ||
| MiFish-0/qPCR-1 | 18.29 | 16.92 | 29.16 | 0.65 | 0.57 | 0.59 | |
| MiFish-1/qPCR-1 | 13.58 | 8.00 | 11.53 | 0.59 | 1.35 | 0.90 | |
| P | <0.001 | <0.001 | <0.001 | <0.001 | <0.001 | <0.001 | |
The data were based on samples after excluding library failure samples. MiFish-0/qPCR-1 samples represent samples in which the qPCR method detected the target fish, whereas the MiFish method did not. MiFish-1/qPCR-1 samples represent samples in which both the qPCR and MiFish methods detected the target fish.