Fig 8. The ORF2 ARM is also able to control the topology of CD4 SP.

A schematic representation of differential permeabilization process with Triton X-100 and Digitonin is shown. Representative images of the differential detection of two epitopes on the ER-membrane associated Calnexin (CNX) in the used permeabilization conditions are shown. H7-T7-IZ cells were transfected with pTM plasmids expressing CD4wt or chimeric C4 and C5 proteins. Twenty-four hours post-transfection, cells were fixed, permeabilized with either Triton X-100 or Digitonin, and processed for CD4 staining (in green). Nuclei are in blue. Representative confocal CD4/DAPI merge images are shown. Blue dots observed in some pictures are DAPI-stained transfected plasmids. A schematic representation of each construct is shown on the left and its predicted topology on the right. Blue and red asterisks correspond to 5R/5A and PSG/3R mutations, respectively. Scale bar, 20μm.