TABLE 1.
Bacterial strains and expression plasmids
| Strain or plasmid | Relevant genotype | Reference or source |
|---|---|---|
| Strains | ||
| V. cholerae | ||
| KSK618 | CG842 tcpP-lacZ (classical Ogawa Smr) | 33 |
| KSK805 | KSK618 ΔaphA1 ΔaphB1 | 21 |
| KSK725 | KSK262 tcpP-lacZ (El Tor Inaba Smr) | 21 |
| GK184 | KSK725 ΔaphA1 ΔaphB1 | This work |
| GK250 | KSK725 ΔtcpPH promoter | This work |
| GK300 | KSK618 ΔtcpPH promoter | This work |
| GK318 | GK300 with classical tcpPH promoter (C/C control) | This work |
| GK319 | GK300 with El Tor tcpPH promoter (C/E hybrid) | This work |
| GK321 | GK250 with classical tcpPH promoter (E/C hybrid) | This work |
| GK323 | GK250 with El Tor tcpPH promoter (E/E control) | This work |
| GK404 | GK300 with classical tcpPH promoter A→G change at position −65 | This work |
| GK436 | GK250 with El Tor tcpPH promoter G→A change at position −66 | This work |
| KSK218 | CG842 ctx-lacZ Smr Cmr | 32 |
| KSK1019 | KSK218 with TAA codon in tcpP coding region | This work |
| O395 | Classical Ogawa Smr | Laboratory collection |
| KSK1093 | O395 tcpPH promoter A→G change at position −65 | This work |
| C6706 str2 | El Tor Inaba Smr | Laboratory collection |
| KSK1117 | C6706 str2 tcpPH promoter G→A change at position −66 | This work |
| E. coli | ||
| MC1061 | Δ(ara-leu)7697 Δ(lac)X74 | Laboratory collection |
| KSK782 | MC1061 λKSPL1 (tcpP-lacZ wild-type classical) | 21 |
| KSK864 | MC1061 λKSPL2 (tcpP-lacZ wild-type El Tor) | This work |
| GK334 | MC1061 λGK91 (tcpP-lacZ C4/C3 control) | This work |
| GK335 | MC1061 λGK92 (tcpP-lacZ C4/E3 chimera) | This work |
| GK336 | MC1061 λGK93 (tcpP-lacZ E4/C3 chimera) | This work |
| GK337 | MC1061 λGK94 (tcpP-lacZ E4/E3 control) | This work |
| GK370 | MC1061 λGK101 (tcpP-lacZ classical A→G change at position −65) | This work |
| GK372 | MC1061 λGK102 (tcpP-lacZ classical G→T change at position −7) | This work |
| GK407 | MC1061 λGK109 (tcpP-lacZ El Tor G→A −66) | This work |
| GK409 | MC1061 λGK111 (tcpP-lacZ classical C→A change at position −13) | This work |
| GK411 | MC1061 λGK112 (tcpP-lacZ classical A addition at position −19) | This work |
| GK441 | MC1061 λGK117 (tcpP-lacZ class A→C change at position −65) | This work |
| GK442 | MC1061 λGK118 (tcpP-lacZ El Tor G→C change at position −66) | This work |
| GK443 | MC1061 λGK119 (tcpP-lacZ classical A→T change at position −65) | This work |
| GK444 | MC1061 λGK120 (tcpP-lacZ El Tor G→T change at position −66) | This work |
| Expression plasmids | ||
| pKAS107 | pMMB66EH aphA (classical) Apr | 33 |
| pKAS117 | pMMB66EH aphB (classical) Apr | 21 |
| pKAS121 | pBAD-TOPO aphB (classical) Kanr | This work |
| pKAS126 | pBAD-TOPO aphA (classical) Kanr | This work |