TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Reference or source |
|---|---|---|
| Bacterial strains | ||
| E. faecalis | ||
| 10C1 | ATCC 11700 | |
| OG1X | 13 | |
| OG1RF | 10 | |
| OGBKD1 | BkdA− mutant of OG1RF; bkdA::mγδ-200; Kanr | 29 |
| OGBKD2 | BkdC− mutant of OG1RF; bkdC::mγδ-200; Kanr | 29 |
| E. coli | ||
| XL1 Blue | recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac[F′ proAB lacIqZΔM15 Tn10 (Tetr)] | Stratagene |
| Plasmids | ||
| pBluescript | Cloning vector; Apr | Stratagene |
| pUC118/9 | Cloning vector; Apr | 30 |
| pDL278 | Enterococcal and E. coli shuttle plasmid; Spcr | 16 |
| pNZ273 | gusA reporter plasmid; Cmr | 19 |
| pNZ273e | pNZ273 lacking EcoRI site | This work |
| pDW100 | pDL278 containing the 2.0-kb BamHI/HindIII insert of pNZ273e containing gusA; Spcr | This work |
| pDW101 | pDW100 containing a 390-bp BamHI/EcoRI fragment containing the bkd promoter; Spcr | This work |