TABLE 2.
GUS activities for E. faecalis OG1RF(pDW101) grown aerobically in the presence of the branched-chain amino acids and branched-chain α-keto acids
| Carbon source | GUS activity (nmol/min/mg protein)a | Induction ratiob |
|---|---|---|
| None | 78 (4.0) | 1 |
| KIV | 124 (7.1) | 1.6 |
| KIC | 206 (10.2) | 2.6 |
| KMV | 305 (6.5) | 3.9 |
| Valine | 109 (5.8) | 1.4 |
| Isoleucine | 133 (3.2) | 1.7 |
| Leucine | 103 (11.2) | 1.3 |
a
Average of three determinations +/− the standard deviation shown in parentheses.
b
Determined by dividing the enzyme activity in cells grown on M17 medium plus the branched-chain amino acid or α-keto acid by the enzyme activity of cells grown on M17 medium alone.