TABLE 2.
Quantitation of immunogold particles in ultrathin cryosections of B. subtilis wild-type cells before and after heat shock
| Parameter | Valuea
|
|||||
|---|---|---|---|---|---|---|
| ClpP antibody
|
ClpC antibody
|
ClpX antibody
|
||||
| Controlb | Heat shockc | Controlb | Heat shockc | Controlb | Heat shockc | |
| % Cells with inclusions bodies | 0 | 99.2 | 0 | 100 | 0 | 98.3 |
| No. (± SD) of gold particles in cytoplasm per cell | 12.6 ± 6.6 | 17.0 ± 6.9 | 26.4 ± 9.2 | 39.4 ± 11.5 | 15.9 ± 5.1 | 11.9 ± 4.9 |
| No. (± SD) of gold particles at protein aggregates per cell | 0 | 14.0 ± 6.7 | 0 | 30.4 ± 10.2 | 0 | 7.2 ± 4.1 |
a
At least 100 cells were evaluated.
b
Cells were harvested during exponential growth.
c
Cells were harvested 10 min after heat shock at 50°C.