TABLE 3.
Quantitative trait loci (QTL) for RSA-related traits identified from 5 independent tests.
| Traits | QTL | Test | Position (cM) | Flanking Markers | LOD value | PVE (%) | Add | Physical position (Mb) |
| TRL | QTrl.sicau-2SY-2B | T3 | 94 | AX-110580733–AX-109395848 | 4.52 | 11.86 | 133.26 | 46.84–54.29 |
| QTrl.sicau-2SY-4D | T1 | 62 | AX-110527441–AX-110572006 | 4.29 | 15.88 | –93.58 | 14.96–19.59 | |
| T2 | 61 | AX-110527441–AX-110572006 | 8.47 | 25.19 | –85.62 | |||
| T3 | 63 | AX-110527441–AX-110572006 | 6.65 | 15.81 | –151.25 | |||
| T4 | 63 | AX-110527441–AX-110572006 | 4.58 | 15.97 | –95.41 | |||
| T5 | 60 | AX-110527441–AX-110572006 | 8.17 | 26.03 | –144.32 | |||
| BLUP | 62 | AX-110527441–AX-110572006 | 14.62 | 38.48 | –73.91 | |||
| RA | QRa.sicau-2SY-2B.1 | T3 | 95 | AX-110580733–AX-109395848 | 4.22 | 11.54 | 12.80 | 46.84–54.29 |
| QRa.sicau-2SY-2B.2 | BLUP | 82 | AX-111563183–AX-110442271 | 3.76 | 8.36 | 3.82 | 45.78–45.73 | |
| QRa.sicau-2SY-4D | T1 | 61 | AX-110527441–AX-110572006 | 4.51 | 16.25 | –12.44 | 14.96–19.59 | |
| T2 | 61 | AX-110527441–AX-110572006 | 6.50 | 22.60 | –10.76 | |||
| T3 | 62 | AX-110527441–AX-110572006 | 6.88 | 18.39 | –15.88 | |||
| T4 | 63 | AX-110527441–AX-110572006 | 4.52 | 15.33 | –10.70 | |||
| T5 | 61 | AX-110527441–AX-110572006 | 7.50 | 24.12 | –14.89 | |||
| BLUP | 62 | AX-110527441–AX-110572006 | 12.51 | 34.70 | –7.64 | |||
| RD | QRd.sicau-2SY-6B | BLUP | 118 | AX-108816205–AX-111513561 | 3.91 | 9.74 | 0.002 | 156.90–145.79 |
| RV | QRv.sicau-2SY-2B | T3 | 95 | AX-110580733–AX-109395848 | 4.53 | 12.50 | 0.11 | 46.84–54.29 |
| QRv.sicau-2SY-4D | T1 | 61 | AX-110527441–AX-110572006 | 4.54 | 15.97 | –0.13 | 14.96–19.59 | |
| T2 | 61 | AX-110527441–AX-110572006 | 5.36 | 19.34 | –0.11 | |||
| T3 | 62 | AX-110527441–AX-110572006 | 6.75 | 17.35 | –0.13 | |||
| T4 | 62 | AX-110527441–AX-110572006 | 3.94 | 13.45 | –0.10 | |||
| T5 | 61 | AX-110527441–AX-110572006 | 6.30 | 20.75 | –0.12 | |||
| BLUP | 62 | AX-110527441–AX-110572006 | 12.55 | 34.54 | –0.08 | |||
| RT | QRt.sicau-2SY-1B | T5 | 99 | AX-109308955–AX-110970287 | 3.93 | 6.82 | –276.51 | 477.39–476.56 |
| QRt.sicau-2SY-3A | T4 | 120 | AX-110645660–AX-110479868 | 3.57 | 10.24 | –307.51 | 606.20–628.15 | |
| QRt.sicau-2SY-4D | T5 | 59 | AX-94547815–AX-110527441 | 8.05 | 15.56 | –416.61 | 15.42–14.96 | |
| BLUP | 63 | AX-110527441–AX-110572006 | 6.98 | 22.10 | –96.95 | 14.96–19.59 | ||
| RF | QRf.sicau-2SY-2D | T2 | 9 | AX-110604633–AX-111526465 | 4.53 | 10.38 | 204.75 | 70.44–69.86 |
| QRf.sicau-2SY-4D.1 | T5 | 48 | AX-108735064–AX-94547815 | 6.71 | 20.62 | –393.74 | 12.27–15.42 | |
| QRf.sicau-2SY-4D.2 | T1 | 62 | AX-110527441–AX-110572006 | 3.97 | 14.84 | –326.61 | 14.96–19.59 | |
| T2 | 62 | AX-110527441–AX-110572006 | 7.18 | 19.18 | –275.32 | |||
| T3 | 62 | AX-110527441–AX-110572006 | 5.23 | 18.26 | –474.07 | |||
| T4 | 63 | AX-110527441–AX-110572006 | 4.31 | 14.69 | –333.09 | |||
| BLUP | 62 | AX-110527441–AX-110572006 | 12.41 | 32.68 | –221.17 | |||
| QRf.sicau-2SY-6A | BLUP | 149 | AX-108876696–AX-109817601 | 4.58 | 9.66 | 120.16 | 597.85–598.02 | |
| QRf.sicau-2SY-7B | T5 | 163 | AX-108840818–AX-111586468 | 3.79 | 10.74 | 283.78 | 581.75–575.44 | |
| RDW | QRdw.sicau-2SY-4D | T1 | 62 | AX-110527441–AX-110572006 | 4.47 | 16.27 | –0.01 | 14.96–19.59 |
| T5 | 61 | AX-110527441–AX-110572006 | 5.18 | 15.60 | –0.01 | |||
| BLUP | 62 | AX-110527441–AX-110572006 | 9.10 | 26.02 | –0.003 | |||
| SDW | QSdw.sicau-2SY-4B.1 | BLUP | 0 | AX-110928817–AX-111620391 | 3.85 | 7.06 | –0.01 | 38.72–40.61 |
| QSdw.sicau-2SY-4B.2 | T3 | 3 | AX-111573292–AX-111233094 | 4.60 | 17.22 | –0.04 | 40.59–47.63 | |
| QSdw.sicau-2SY-4D | T1 | 62 | AX-110527441–AX-110572006 | 5.12 | 19.12 | –0.03 | 14.96–19.59 | |
| T5 | 63 | AX-110527441–AX-110572006 | 8.03 | 16.07 | –0.04 | |||
| BLUP | 63 | AX-110527441–AX-110572006 | 12.38 | 27.80 | –0.02 | |||
| QSdw.sicau-2SY-5B | BLUP | 0 | AX-108791526–AX-111490382 | 4.04 | 7.43 | –0.01 | 35.16–35.25 | |
| DRS | QDrs.sicau-2SY-4B | BLUP | 52 | AX-109637078–AX-109861624 | 7.12 | 6.66 | 0.01 | 519.23–529.15 |
| QDrs.sicau-2SY-5A.1 | BLUP | 17 | AX-109512380–AX-111572088 | 8.11 | 7.72 | –0.01 | 581.77–585.45 | |
| QDrs.sicau-2SY-5A.2 | T3 | 24 | AX-110373981–AX-110404585 | 3.41 | 12.09 | 0.02 | 587.31–587.07 | |
| BLUP | 24 | AX-110373981–AX-110404585 | 16.69 | 18.56 | 0.01 | |||
| QDrs.sicau-2SY-5D | T4 | 40 | AX-108968976–AX-108881619 | 4.12 | 15.12 | 0.02 | 54.93–59.50 | |
| QDrs.sicau-2SY-7A | T3 | 41 | AX-110030320–AX-111499495 | 3.31 | 11.75 | –0.02 | 629.63–632.34 | |
| RN | QRn.sicau-2SY-5B | T5 | 14 | AX-110402737–AX-110586945 | 3.74 | 13.28 | –0.52 | 586.59–510.41 |
| QRn.sicau-2SY-6B | T4 | 6 | AX-89696866–AX-109900030 | 3.88 | 13.50 | –0.56 | 666.87–680.00 |
LOD logarithm of odds, PVE phenotype variance explained, Add additive effect of a QTL, TRL total root length, RA root area, RV root volume, RD root diameter, RT root tips, RF root forks, RDW root dry weight, SDW shoot dry weight, DRS dry root–shoot ratio, RN root number, BLUP best linear unbiased prediction; T represents Test; Positive value of Add indicates that alleles from 20828 are increasing the trait scores, and negative value of Add indicates that alleles from SY95-71 are increasing the trait scores; The physical position of each marker came from CS v2.1 genome.