Skip to main content
. 2022 Aug 22;11:e79957. doi: 10.7554/eLife.79957

Figure 7. IL-12 and TGFβ1 are critical for dendritic cell (DC) piezo1-dependent T cell differentiation in promoting cancer growth.

(A) MC38 tumor cells were implanted subcutaneously in WT and Piezo1-/- mice (n=10), IL-12 100 ng or anti-TGFβ1 mAb 200 ng per mouse in 50 µl volume or vehicle (PBS) was locally injected into tumor once a week and tumor size was measured every 5 days for 40 days. (B) Intracellular staining of IFNγ, IL-17A, and Foxp3 expression in CD4+ T cells from the tumor of WT and Piezo1-/- tumor-bearing mice at day 40. (C) Piezo1 mRNA expression of human DCs with the indicated treatment (lipopolysaccharide [LPS], 10 ng/ml or conditioned with 2 or 50 kPa hydrogels plate or LPS+50 kPa hydrogels plate). Levels in the vehicle group were set to 1. (D–E) IL-12p70 (D) and TGFβ1 (E) production of human DCs treated by LPS (10 ng/ml) for 5 hr. (F–H) Human DCs pulsed with LPS (10 ng/ml) were cocultured with human T cells for 5 days, in the absence or presence of Yoda1 (25 μM). The intracellular staining of IFNγ and Foxp3 in T cells. ***p<0.001 compared with the indicated groups. Data are representative of three independent experiments (mean ± s.d.; n=3–4). ***p<0.001, compared with the indicated groups.

Figure 7.

Figure 7—figure supplement 1. Dendritic cell (DC) Piezo1 controls the differentiation of TH1 and Treg cells in cancer.

Figure 7—figure supplement 1.

Proposed model of how Piezo1 in DCs responses to inflammatory stimuli or stiffness signals to regulate the differentiation of TH1 and Treg cell populations in regulating cancer growth.