| Genetic reagent (Mus musculus) |
Mouse: C57BL/6J (CD45.1 and CD45.2) |
Jackson Laboratory |
RRID:IMSR_JAX:000664
|
|
| Genetic reagent (Mus musculus) |
Mouse: Piezo1flox/flox
|
Jicuiyaokang company of China |
|
|
| Genetic reagent (Mus musculus) |
Mouse: Cd11c-Cre |
Nanfangmoshi biological company of China |
|
|
| Genetic reagent (Mus musculus) |
Mouse: Piezo1-/-
|
Jicuiyaokang company of China |
|
|
| Genetic reagent (Mus musculus) |
Mouse: Sirt1flox/flox
|
Jackson Laboratory |
|
|
| Genetic reagent (Mus musculus) |
Mouse: Hif1aflox/flox
|
Jackson Laboratory |
|
|
| Genetic reagent (Mus musculus) |
Mouse: Cd4-Cre |
Jackson Laboratory |
|
|
| Genetic reagent (Mus musculus) |
Mouse: OTII TCR-transgenic mice |
Jicuiyaokang company of China |
|
|
| Biological sample (Mus musculus) |
Primary mouse splenocyte cells |
Beijing Normal University |
|
Freshly isolated from mice |
| Biological sample (Mus musculus) |
Primary mouse bone marrow cells |
Beijing Normal University |
|
Freshly isolated from mice |
| Biological sample (Mus musculus) |
Primary mouse serum |
Beijing Normal University |
|
Freshly isolated from mice |
| Cell line |
Mouse colon cancer cell line MC-38 |
China cell bank ATCC Center |
MC38 |
|
| Cell line |
Mouse melanoma cell line B16.F10 |
China cell bank ATCC Center |
B16.F10 |
|
| Cell line |
Human DC |
Lonza |
CC-2701 |
|
| Cell line |
Human cord blood CD4+ T cells |
Lonza |
2C-200 |
|
| Antibody |
Rat monoclonal anti-mouse CD11c FITC |
Thermo Fisher Scientific |
Cat # MHCD11C01; RRID: AB_10373970
|
FCS (1:100) |
| Antibody |
Rat monoclonal anti-mouse CD11c APC eFluor780 |
Thermo Fisher Scientific |
Cat# 47-0116-42 |
FCS (1:100) |
| Antibody |
Rat monoclonal anti-mouse CD11c PE |
Thermo Fisher Scientific |
Cat# 12-0114-81 |
FCS (1:100) |
| Antibody |
Rat monoclonal anti-mouse CD4 APC-Cy7 |
BD Bioscience |
Cat#130-109-536 |
FCS (1:100) |
| Antibody |
Rat monoclonal anti-mouse CD8α FITC |
Thermo Fisher Scientific |
Cat# 11-0081-82 |
FCS (1:100) |
| Antibody |
Rat monoclonal anti-mouse CD11b FITC |
Thermo Fisher Scientific |
Cat# 11-0112-82 |
FCS (1:100) |
| Antibody |
Rat monoclonal anti-mouse Ly6G PE |
Thermo Fisher Scientific |
Cat# 12-9668-82 |
FCS (1:100) |
| Antibody |
Rat monoclonal anti-mouse F4/80 PE |
Thermo Fisher Scientific |
Cat# 12-4801-82 |
FCS (1:100) |
| Antibody |
Rat monoclonal anti-mouse CD19 PE |
Thermo Fisher Scientific |
Cat# 12-0199-42 |
FCS (1:100) |
| Antibody |
Rat monoclonal anti-mouse TCR FITC |
Thermo Fisher Scientific |
Cat# TCR2730 |
FCS (1:100) |
| Antibody |
Rat monoclonal anti-mouse CD44 FITC |
Thermo Fisher Scientific |
Cat# 11-0441-82 |
FCS (1:100) |
| Antibody |
Rat monoclonal anti-mouse CD62L APC |
Thermo Fisher Scientific |
Cat# 11-0621-82 |
FCS (1:100) |
| Antibody |
Rat monoclonal anti-mouse CD80 APC |
Thermo Fisher Scientific |
Cat# 17-0801-82 |
FCS (1:100) |
| Antibody |
Rat monoclonal anti-mouse CD54 FITC |
Thermo Fisher Scientific |
Cat# 17-0549-42 |
FCS (1:100) |
| Antibody |
Rat monoclonal anti-mouse MHCII APC |
Thermo Fisher Scientific |
Cat# 17-5320-82 |
FCS (1:100) |
| Antibody |
Rat monoclonal anti-mouse CD45 APC |
Thermo Fisher Scientific |
Cat# 17-0459-42 |
FCS (1:200) |
| Antibody |
Rat monoclonal anti-mouse NK1.1 PE |
Thermo Fisher Scientific |
Cat# 25-5941-82 |
FCS (1:200) |
| Antibody |
Rat monoclonal Anti-mouse CCR7 APC |
Thermo Fisher Scientific |
Cat# A18389 |
FCS (1:200) |
| Antibody |
Mouse monoclonal anti-mouse PDL1 PE |
Thermo Fisher Scientific |
Cat# 12-5982-82 |
FCS (1:200) |
| Antibody |
Mouse monoclonal anti-mouse PDL2 PE |
Thermo Fisher Scientific |
Cat# 12-5986-82 |
FCS (1:200) |
| Antibody |
Mouse monoclonal anti-mouse IFNγ PE |
Thermo Fisher Scientific |
Cat# 12-7311-82 |
FCS (1:100) |
| Antibody |
Mouse monoclonal anti-mouse IL-4 PE |
Thermo Fisher Scientific |
Cat# 12-7041-82 |
FCS (1:100) |
| Antibody |
Rat monoclonal anti-mouse Foxp3 PE |
Thermo Fisher Scientific |
Cat# 12-5773-82 |
FCS (1:100) |
| Antibody |
Rat monoclonal anti-mouse IL-12p40 |
Thermo Fisher Scientific |
Cat# 12-7123-82 |
FCS (1:100) |
| Antibody |
Rat monoclonal anti-mouse TGFβ1 |
Thermo Fisher Scientific |
Cat# 12-9829-42 |
FCS (1:100) |
| Commercial assay or kit |
Fixation/Permeabilization Solution Kit |
BD Bioscience |
Cat# 554714 |
|
| Commercial assay or kit |
RNeasy Mini Kit |
Qiagen |
Cat# 74106 |
|
| Commercial assay or kit |
FastQuant RT Kit |
Tiangen |
Cat# KR106-02 |
|
| Commercial assay or kit |
Mouse IL-12 p70 Quantikine ELISA Kit |
R&D Systems |
Cat# M1270 |
|
| Commercial assay or kit |
Mouse TGF-beta 1 DuoSet ELISA |
R&D Systems |
Cat# DY1679 |
|
| Commercial assay or kit |
SuperReal PreMix Plus SYBR Green |
Tiangen |
Cat# FP205-02 |
|
| Chemical compound, drug |
Cyclosporin A |
Signa-Aldrich |
Cat# 59865-13-3 |
|
| Chemical compound, drug |
Yoda1 |
MCE |
Cat# HY-18723 |
|
| Chemical compound, drug |
11R-VIVIT |
MCE |
Cat# HY-P1430 |
|
| Chemical compound, drug |
Gadolinium chloride |
Sigma-Aldrich |
Cat# 19423-81-5 |
|
| Chemical compound, drug |
Ruthenium red |
Sigma-Aldrich |
Cat# 12790-48-6 |
|
| Chemical compound, drug |
2-Deoxy-D-glucose |
Sigma-Aldrich |
Cat# 29702-43-0 |
|
| Chemical compound, drug |
Diethyl succinate |
Sigma-Aldrich |
Cat# 123-25-1 |
|
| Chemical compound, drug |
IL-4 |
R&D Systems |
Cat# 204-IL |
|
| Chemical compound, drug |
IL-12 |
Peprotech |
Cat# 210–12 |
|
| Chemical compound, drug |
Fibronectin |
Sigma-Aldrich |
Cat# ECM001 |
|
| Chemical compound, drug |
Carbonyl cyanide-4-[trifluoromethoxy] phenylhydrazone |
Sigma-Aldrich |
Cat# SML2959 |
|
| Chemical compound, drug |
Rotenone |
Sigma-Aldrich |
Cat# R8875 |
|
| Chemical compound, drug |
Fura2 AM |
Sigma-Aldrich |
Cat# 47989 |
|
| Chemical compound, drug |
LPS |
Sigma-Aldrich |
Cat# 2630 |
|
| Chemical compound, drug |
Freund’s Adjuvant, Complete |
Sigma–Aldrich |
Cat# F5881 |
|
| Chemical compound, drug |
PMA |
Sigma-ALdrich |
Cat# P8139 |
|
| Chemical compound, drug |
Ionomycin |
Sigma-Aldrich |
Cat# I0634 |
|
| Chemical compound, drug |
GM-CSF |
R&D Systems |
Cat# 7954GM-010/CF |
|
| Chemical compound, drug |
Collagenase D |
Worthington |
Cat# LS005273
|
|
| Chemical compound, drug |
Deoxyribonuclease I |
Beyotime Biotechnology |
Cat# D7076 |
|
| Chemical compound, drug |
Percoll |
GE Health |
Cat# 17-0891-01 |
|
| Software, algorithm |
Adobe Illustrator |
Adobe |
RRID:SCR_010279
|
|
| Other |
One-micrometer latex microspheres |
Polysciences, Inc |
Cat# 19821 |
|
| Other |
7-AAD |
BD Bioscience |
Cat# 559925 RRID: AB_2869266
|
A dye for identifying cell death |
| Other |
Streptavidin-APC/eFluor 780 |
Thermo Fisher Scientific |
Cat# 47-4317-82; RRID:AB_10366688
|
A secondary antibody for indirect staining to detect biotinylated primary antibody; FCS (1:500) |
| Other |
Streptavidin-eFluor 450 |
Streptavidin-eFluor 450 |
Cat# 48-4317-82; RRID:AB_10359737
|
A secondary antibody for indirect staining to detect biotinylated primary antibody; FCS (1:500) |
| Other |
SYLGARD 527 A&B Silicone Dielectric Gel |
Dow |
|
Silicone Dielectric Gel |
