Appendix 1—table 1. Primer sequences for genotyping and qRT-PCR analysis.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Sequence-based reagent | Kras.in3.F | This paper | PCR primers | TTGGTGTACATCACTAGGCTTCA |
| Sequence-based reagent | Kras.in3.R | This paper | PCR primers | TGGAAAGAGTAAAGTGTGGTGGT |
| Sequence-based reagent | Kras.neo.F | This paper | PCR primers | GTGGGCTCTATGGCTTCTGA |
| Sequence-based reagent | KrasCOM5.F | This paper | PCR primers | CTTCCATTTGTCACGTCCTGC |
| Sequence-based reagent | KrasCOM5.R | This paper | PCR primers | TCTTCGGTGGAAACAACGGT |
| Sequence-based reagent | F-LSL | This paper | PCR primers | TAGTCTGTGGGACCCCTTTG |
| Sequence-based reagent | R-LSL | This paper | PCR primers | GCCTGAAGAACGAGATCAGC |
| Sequence-based reagent | KRASOP.A2 | This paper | PCR primers | CTAGCCACCATGGCTTGAGT |
| Sequence-based reagent | KRASOP.B | This paper | PCR primers | GTAATCACAACAAAGAGAATGCAG |
| Sequence-based reagent | LSL-F | This paper | PCR primers | GGGGAACCTTTCAGGCTTA |
| Sequence-based reagent | KrasRAR.F | This paper | PCR primers | TATGCGTACGGGTGAAGGTT |
| Sequence-based reagent | KrasRAR.R | This paper | PCR primers | GCAGAGCACAGACTCACGTC |
| Sequence-based reagent | F-CC10 WT | Xu et al., 2012 | PCR primers | ACTCACTATTGGGGGTGTGG |
| Sequence-based reagent | R-CC10 WT | Xu et al., 2012 | PCR primers | GGAGGACTTGTGGATCTTG |
| Sequence-based reagent | F-Cre | Xu et al., 2012 | PCR primers | TCGATGCAACGAGTGATGAG |
| Sequence-based reagent | R-Cre | Xu et al., 2012 | PCR primers | TTCGGCTATAGGTAACAGGG |
| Sequence-based reagent | F-Rosa-01 | Xu et al., 2012 | PCR primers | CACTTGCTCTTCCAAAGTCG |
| Sequence-based reagent | R-Rosa-02B | Xu et al., 2012 | PCR primers | TAGTCTAACTCGCGACACTG |
| Sequence-based reagent | F-CAG-02B | Xu et al., 2012 | PCR primers | GTTATGTAACGCGGAACTCC |
| Sequence-based reagent | R26R-univF | Jackson Laboratory | PCR primers | AAAGTCGCTCTGAGTTGTTAT |
| Sequence-based reagent | R26R-wtR | Jackson Laboratory | PCR primers | GGAGCGGGAGAAATGGATATG |
| Sequence-based reagent | CreER-R1 | Jackson Laboratory | PCR primers | CCTGATCCTGGCAATTTCG |
| Sequence-based reagent | GLI1-F | This paper | PCR primers | CCCATAGGGTCTCGGGGTCTCAAAC |
| Sequence-based reagent | GLI1-R | This paper | PCR primers | GGAGGACCTGCGGCTGACTGTGTAA |
| Sequence-based reagent | CDH1-F | This paper | PCR primers | GTCTCCTCATGGCTTTGC |
| Sequence-based reagent | CDH1-R | This paper | PCR primers | CTTTAGATGCCGCTTCAC |
| Sequence-based reagent | TWIST1-F | This paper | PCR primers | AGCGGGTCATGGCTAACG |
| Sequence-based reagent | TWIST1-R | This paper | PCR primers | GGACCTGGTACAGGAAGTCGA |
| Sequence-based reagent | ZEB2-F | This paper | PCR primers | GAGCTTGACCACCGACTC |
| Sequence-based reagent | ZEB2-R | This paper | PCR primers | TTGCAGGACTGCCTTGAT |
| Sequence-based reagent | SOX5-F | This paper | PCR primers | ATTGTGCAGTCCCACAGGTTG |
| Sequence-based reagent | SOX5-R | This paper | PCR primers | CTGCCTTTAGTGGGCCAGTG |
| Sequence-based reagent | IL6-F | This paper | PCR primers | CCGGAGAGGAGACTTCACAG |
| Sequence-based reagent | IL6-R | This paper | PCR primers | CAGAATTGCCATTGCACAAC |
| Sequence-based reagent | IL10-F | This paper | PCR primers | GGTTGCCAAGCCTTATCGGA |
| Sequence-based reagent | IL10-R | This paper | PCR primers | ACCTGCTCCACTGCCTTGCT |
| Sequence-based reagent | TNFa-F | This paper | PCR primers | CCCCAAAGGGATGAGAAGTT |
| Sequence-based reagent | TNFa-R | This paper | PCR primers | GTGGGTGAGGAGCACGTAGT |
| Sequence-based reagent | CCL2-F | This paper | PCR primers | AGGTCCCTGTCATGCTTCTG |
| Sequence-based reagent | CCL2-R | This paper | PCR primers | TCTGGACCCATTCCTTCTTG |
| Sequence-based reagent | LIF-F | This paper | PCR primers | AATGCCACCTGTGCCATACG |
| Sequence-based reagent | LIF-R | This paper | PCR primers | CAACTTGGTCTTCTCTGTCCCG |
| Sequence-based reagent | TNFSF9-F | This paper | PCR primers | GCAAGCAAAGCCTCAGGTAG |
| Sequence-based reagent | TNFSF9-R | This paper | PCR primers | TCCAGGAACGGTCCACTAAC |
| Sequence-based reagent | IFNg-F | This paper | PCR primers | CGGGAGGTGCTGCTGATGG |
| Sequence-based reagent | IFNg-R | This paper | PCR primers | AGGGACAGCCTGTTACTACC |
| Sequence-based reagent | FOS-F | This paper | PCR primers | CCTGCCCCTTCTCAACGAC |
| Sequence-based reagent | FOS-R | This paper | PCR primers | GCTCCACGTTGCTGATGCT |
| Sequence-based reagent | FOSL2-F | This paper | PCR primers | GAGTCCTACTCCAGCGGTG |
| Sequence-based reagent | FOSL2-R | This paper | PCR primers | GACTGTAGGGATGTGAGCGT |
| Sequence-based reagent | DUSP6-F | Li and Counter, 2021 | PCR primers | ACTTGGACGTGTTGGAAGAGT |
| Sequence-based reagent | DUSP6-R | Li and Counter, 2021 | PCR primers | GCCTCGGGCTTCATCTATGAA |
| Sequence-based reagent | EGR1-F | Li and Counter, 2021 | PCR primers | CCTGACCACAGAGTCCTTTTCT |
| Sequence-based reagent | EGR1-R | Li and Counter, 2021 | PCR primers | AGGCCACTGACTAGGCTGA |
| Sequence-based reagent | FOSL1-F | Li and Counter, 2021 | PCR primers | CAGGAGTCATACGAGCCCTAG |
| Sequence-based reagent | FOSL1-R | Li and Counter, 2021 | PCR primers | GCCTGCAGGAAGTCTGTCAG |
| Sequence-based reagent | ACTIN-F | Li and Counter, 2021 | PCR primers | CGTGAAAAGATGACCCAGATCATGT |
| Sequence-based reagent | ACTIN-R | Li and Counter, 2021 | PCR primers | CGTGAGGGAGAGCATAGCC |