Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Antibody | Anti-FLAG (mouse monoclonal) | Sigma | F1804 | WB (1:1000) |
| Antibody | Anti-KRAS (mouse monoclonal) | Santa Cruz Biotechnology | sc-30 | WB (1:500) |
| Antibody | Anti-βTubulin (mouse monoclonal) | Sigma | T5201 | WB (1:10,000) |
| Antibody | Anti-SV40 large T antigen (rabbit monoclonal) | Cell Signaling | 15729 | WB (1:1000) |
| Strain, strain background (Escherichia coli) | STBL3 | Thermo Fisher Scientific | C737303 | Chemically competent cells |
| Commercial assay or kit | FuGENE 6 Transfection Reagent | Promega | E2691 | |
| Commercial assay or kit | DC Protein Assay | Bio-Rad | 5000112 | |
| Commercial assay or kit | DNeasy Blood and Tissue DNA extraction Kit | QIAGEN | 69504 | |
| Chemical compound, drug | Tamoxifen | Sigma-Aldrich | T5648-5G | |
| Commercial assay or kit | RNAeasy Kit | QIAGEN | 74104 | |
| Commercial assay or kit | RNase-Free DNase Set | QIAGEN | 79254 | |
| Commercial assay or kit | GenPoint kit | Agilent | K062011-2 | |
| Commercial assay or kit | MycoAlert PLUS Mycoplasma detection kit | Lonza | LT07-703 | |
| Antibody | Anti-rabbit IgG antibody | Vector Laboratories | BA-1000-1.5 | RPPA |
| Antibody | Anti-mouse IgG antibody | Vector Laboratories | BA-9200-1.5 | RPPA |
| Commercial assay or kit | Active Ras Detection Kit | Cell Signaling | 8821 | |
| Commercial assay or kit | Ras GTPase ELISA Kit | Abcam | ab134640 | |
| Cell line (Homo sapiens) | HEK-HT | Counter et al., 1992 | Cell line created and maintained in C. Counter lab, validated by immunoblot for SV40 Large T antigen | Cultured in DMEM and 10% FBS Tested negative for mycoplasma |
| Cell line (Mus musculus) | MEF derived from KrasLSL-natG12D/+ mice | This paper | Validated by genotyping PCR | Cultured in DMEM and 10% FBS Tested negative for mycoplasma |
| Cell line (M. musculus) | MEF derived from KrasLSL-natQ61R/+ mice | This paper | Validated by genotyping PCR | Cultured in DMEM and 10% FBS Tested negative for mycoplasma |
| Cell line (M. musculus) | MEF derived from KrasLSL-comG12D/+ mice | This paper | Validated by genotyping PCR | Cultured in DMEM and 10% FBS Tested negative for mycoplasma |
| Cell line (M. musculus) | MEF derived from KrasLSL-comQ61R/+ mice | This paper | Validated by genotyping PCR | Cultured in DMEM and 10% FBS Tested negative for mycoplasma |
| Strain, strain background (M. musculus) | Kras LSL-natG12D/+ | This paper | Generated in Counter Lab | |
| Strain, strain background (M. musculus) | Kras LSL-natQ61R/+ | This paper | Generated in Counter | |
| Strain, strain background (M. musculus) | Kras LSL-comG12D/+ | This paper | Generated in Counter Lab | |
| Strain, strain background (M. musculus) | Kras LSL-comQ61R/+ | This paper | Generated in Counter Lab | |
| Strain, strain background (M. musculus) | Kras LSL-rareG12D/+ | This paper | Generated in Counter Lab | |
| Strain, strain background (M. musculus) | ACTBFLPe/FLPe | Jackson Laboratory | 003800 | |
| Strain, strain background (M. musculus) | CC10 CreER/CreER ; Rosa26 CAG-fGFP/CAG- fGFP | Xu et al., 2012 | ||
| Strain, strain background (M. musculus) | Rosa26 CreERT2/CreERT2 | Jackson Laboratory | 008463 | |
| Recombinant DNA reagent | pcDNA3.1 | Thermo Fisher Scientific | V79020 | Mammalian expression vector backbone |
| Recombinant DNA reagent | pcDNA3.1+FLAG-KrasnatG12D | This study | Generated in Counter Lab | |
| Recombinant DNA reagent | pcDNA3.1+FLAG-KrasnatQ61R | This study | Generated in Counter Lab | |
| Recombinant DNA reagent | pcDNA3.1+FLAG-KrascomG12D | This study | Generated in Counter Lab | |
| Recombinant DNA reagent | pcDNA3.1+FLAG-KrascomQ61R | This study | Generated in Counter Lab | |
| Recombinant DNA reagent | pBABE-neo largeTcDNA | Hahn et al., 2002 | Addgene #1780 | |
| Recombinant DNA reagent | MSCV-Cre-Hygro | Wang et al., 2010 | Addgene #34565 | |
| Software, algorithm | ImageJ version 1.52k with Java 1.8.0_172 | Schneider et al., 2012 | https://imagej.nih.gov/ij/ | |
| Software, algorithm | Image Lab | Bio-Rad | https://www.bio-rad.com/en-us/product/image-lab-software | |
| Software, algorithm | GraphPad Prism v8 | GraphPad | https://www.graphpad.com/ | |
| Software, algorithm | bcl2fastq | Illumina | https://support.illumina.com/sequencing/sequencing_software/bcl2fastq-conversion-software.html | |
| Software, algorithm | STAR RNA-Seq alignment tool v2.7.8a | Dobin et al., 2013 | https://github.com/alexdobin/STAR | STAR (RRID:SCR_004463) |
| Software, algorithm | DESeq2 | Love et al., 2014 | http://www.bioconductor.org/packages/release/bioc/html/DESeq2.html | |
| Software, algorithm | GSEA | Mootha et al., 2003 | https://www.gsea-msigdb.org/gsea/ | |
| Software, algorithm | R Studio | R Development Core Team, 2020 | https://www.R-project.org | |
| Software, algorithm | MicroVigene Software Version 5.1.0.0 | VigeneTech | http://www.vigenetech.com/Protein.htm | |
| Software, algorithm | BioRender | http://www.biorender.com |