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. 2022 Aug 24;12:979748. doi: 10.3389/fonc.2022.979748

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Copyright © 2022 Xu, Abramov, Belykh, Mignucci-Jiménez, Park, Eschbacher and Preul

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Bar chart showing mean (SD) brightness and contrast of images acquired at different time intervals after fluorescein sodium (FNa) dosing. (A) Comparison of images acquired later than 120 min after FNa dosing in both studies; the mean brightness (in vivo vs. ex vivo: 105.1 [18.9] vs. 72.4 [24.9], p < 0.001) and contrast (in vivo vs. ex vivo: 41.7 [8.7] vs. 27.1 [8.5], p < 0.001) of the in vivo images are significantly higher than those of the ex vivo images. (B) Compared to the images acquired earlier than 120 minutes after FNa dose, the images acquired later than 120 minutes had a slight decrease in brightness (112.2 [27.0] to 105.1 [18.9], p < 0.001) and contrast (44.74 [8.0] to 41.07 [8.6], p < 0.001). Values are reported in optical density units defined by Fiji software. Asterisk indicates p < 0.001. Used with permission from Barrow Neurological Institute, Phoenix, Arizona.