FIG. 2.

(A) Utilization of the same transcription start site in the sbo-alb promoter region under aerobic and anaerobic conditions. Tenfold less primer extension reaction mixture was applied to lane 6 (RNA from anaerobic cultures) than was applied to lane 5 (RNA from aerobic cultures). Sequencing reactions G, A, T, and C are in lanes 1, 2, 3, and 4, respectively. (B) Nucleotide sequence of the sbo-alb promoter region and similarity to the regulatory region of the resA operon. The ATG start site of the sboA gene and its ribosome-binding site (SD) are shown. The −10 and −35 regions are indicated, as is the transcription start site (∗), and a region of approximate dyad symmetry upstream of the −35 sequence is marked with arrows beneath the nucleotide sequence. A broken bar above the nucleotide sequence extending from −36 to −62 marks the sequence identity between the resA and sbo-alb regulatory regions. The sequence above the bar indicates the nucleotide residues of the corresponding region in the resA promoter region.