TABLE 1.
Strains, plasmids, and oligonucleotides
| Strain, plasmid, or oligonucleotide | Description | Source and/or reference |
|---|---|---|
| Strains | ||
| R. prowazekii Madrid E | Ems | 7 |
| E. coli XL1-Blue | recA1 endA1 gvrA96 thi-1 hsdR17 supE44 relA1 lac [F′ proAB lacIqZΔM15 Tn10 (Tetr)] | Stratagene (5) |
| Plasmids | ||
| pMOB | Apr | Gold BioTechnology (17)a |
| pAT72 | Emr | 2 |
| pMW1041 | Aps Emr | This study |
| pMW264 | pBR322 with inserted 1,881-bp R. prowazekii EcoRV fragment containing the gltA gene | 23 |
| pMW1047 | pMW1041 with inserted 1,881-bp EcoRV fragment from pMW264 | This study |
| Deoxyoligonucleotidesb | ||
| DW318 | CATGAACCTTACACGTTGAGC (gltA) | DNAgencyc |
| DW331 | CACCTAATGCTACAACTCGGG (ereB) | DNAgency |
| DW337 | GGAGATACCCGAGTTGTAGC (ereB) | Life Technologiesd |
| DW338 | GGCTGCCTGTGATGTGGAG (ereB) | Life Technologies |
a
Gold Biotechnology, St. Louis, Mo.
b
All sequences are written 5′-3′.
c
DNAgency, Ashton, Pa.
d
Life Technologies, Rockville, Md.