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. 2022 Aug 17;609(7926):354–360. doi: 10.1038/s41586-022-05105-1

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 8 — (a–n) Naive CD45.1 mice were lethally irradiated and reconstituted using a mixture of Myb^fl/flCd4^Cre (Myb-cKO) and Cd4^Cre or littermate Myb^fl/fl control (Ctrl) bone marrow. Chimeric mice were subsequently infected with LCMV-Docile and analysed at the indicated time points after infection. Quantification showing the frequencies of (a) polyclonal antigen-specific gp33⁺ cells among Myb-cKO and control CD8⁺ T cells at 8 dpi. (b) Flow cytometry plots and quantification of IFNγ⁺ cells among Myb-cKO and control CD8⁺ T cells after peptide restimulation in vitro at 8 dpi. (c) Quantification of GZMB expression among gp33⁺ cells of the indicated genotypes. (d, e) Flow cytometry plots and quantification showing the frequencies of (d) Ki67⁺ cells and (e) annexin-V⁺ cells among Myb-cKO and control antigen-responsive CD8⁺ T cells. (f, g) Flow cytometry plots and quantification showing the frequencies of TCF1⁺ T_PEX cells among antigen-specific T cells (f) and CD62L⁺ cells among T_PEX cells (g) in Myb-cKO and control compartments at 8 dpi. (h) Flow cytometry plots showing the frequencies of T_PEX cells among gp33⁺ cells at 49 dpi. (i–j) Flow cytometry plots (i) and quantification (j) showing kinetics of splenic polyclonal PD-1⁺ T_PEX cells among Myb-cKO and control CD8⁺ T cells after infection. (k–l) Flow cytometry plots (k) and quantification (l) showing the frequencies of the entire antigen-responsive PD-1⁺ cell compartment among Myb-cKO and control CD8⁺ T cells at indicated time points after infection. (m, n) Flow cytometry plots and quantifications showing the frequencies of Ki67⁺ cells among Myb-cKO and control polyclonal T_PEX (m) and T_EX (n) cells at indicated time points after infection. GMFI, geometric mean fluorescence intensity. Dots in graphs represent individual mice; box plots indicate range, interquartile and median. Symbols and error bars represent mean and s.e.m., respectively. All data are representative of two independent experiments. P values are from two-tailed unpaired t-tests (a–g) and Mann–Whitney tests (j–n); P > 0.05, not significant (n.s.).

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