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. 2022 Aug 17;609(7926):354–360. doi: 10.1038/s41586-022-05105-1

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 12 — (a–f) Congenically marked PD-1-deficient (Pdcd1^−/−) and control P14 T cells were transferred to naive mice, which were subsequently infected with LCMV-Docile. Splenic P14 T cells were analysed at 7 dpi. (a) Schematic of the experimental set-up. (b) P14 T cell frequencies and numbers of indicated genotypes. (c) Flow cytometry plots and frequencies of T_PEX (Ly108^hiTIM-3^lo) and T_EX (Ly108^loTIM-3^hi) cells. (d) Box plots show frequencies and numbers of CD62L⁺ T_PEX cells among control and PD-1-deficient P14 cells. Flow cytometry plots and box plots show (e) frequencies of KIT⁺ T_PEX cells and (f) numbers of KIT⁺ T_PEX and T_EX cells per spleen. (g–k) Wild-type mice were infected with LCMV-Docile and treated with anti-PD-L1 at 200 μg/mouse at 1, 3 and 5 dpi. Splenic CD8⁺ T cells were analysed at 6 dpi. (g) Schematic of the experimental set-up. (h) Flow cytometry plots and quantification showing the frequencies of PD-1⁺ cells among splenic CD8⁺ T cells. (i–j) Flow cytometry plots (i) and quantification (j) showing expression of CD62L among polyclonal T_PEX cells (Ly108^hiTIM-3^lo). (k) Quantification showing the population sizes of CD62L⁺ T_PEX, CD62L⁻ T_PEX and T_EX cells among total CD8⁺ T cells in untreated and anti-PD-L1-treated mice. (l–p) Wild-type mice were infected with LCMV-Docile and treated with anti-PD-L1 at 200 μg/mouse at 21, 23, 25, 27 and 29 dpi. Splenic CD8⁺ T cells were analysed at 31 dpi. (l) Schematic of the experimental set-up. (m–n) Flow cytometry plots (m) and quantification (n) showing the frequencies of the PD-1⁺ cells among splenic CD8⁺ T cells. (o) Flow cytometry plots and quantification showing the expression of CD62L among polyclonal T_PEX cells (Ly108^hiTIM-3^lo). (p) Quantification showing the population sizes of CD62L⁺ T_PEX, CD62L⁻ T_PEX and T_EX cells among total CD8⁺ T cells in untreated and anti-PD-L1-treated mice. Dots in graphs represent individual mice; box plots indicate range, interquartile and median. Data are representative of at least two independent experiments. P values are from two-tailed unpaired t-tests; P > 0.05, not significant (n.s.).

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