Extended Data Table 1.

Summary of key properties of clinch-contact mutants

^aAtomic force microscopy (AFM) (Extended Data Fig. 4a) and negative stain transmission electron microscopy (TEM) imaging of bacterial cells (Fig. 4c and Extended Data Fig. 4b). The number of plus signs indicates the amount of pili: ++++, amount similar to that of the wild type (WT); + to +++, a reduced number of WT-like pili; –/+, pilus-like structures observed on some bacterial cells but not others; –, no pili observed. NT, not tested. The total mass of extracted pilus material was evaluated by Western blotting using the anti-CsuA/Bsc antibody (see Extended Data Fig. 5b).

^bCsu-mediated biofilms on plastics with or without anti-tip (αE_N) antibody. The density of uninduced cells was subtracted from the biofilm cell density data (Fig. 4d and Extended Data Fig. 7a) and normalized to the biofilm density of WT cells in the absence of αE_N. The result of biofilm inhibition by αE_N diluted 1:2500 times is shown (see Extended Data Fig. 7a for the full set of data).

^cCsuC-assisted assembly of CsuA/B (Fig. 4b and Extended Data Fig. 6d). ++++, WT-like polymerization efficiency; +++, most subunits form DSC-polymers; -, not detected due to poor yield.

^dRelative level of WT and mutant CsuA/Bsc in the E. coli periplasm (Fig. 4a and Extended Data Fig. 6a).

^eMelting temperature (T_m) of WT and mutant CsuA/Bsc obtained by measuring circular dichroism at different temperatures (Extended Data Fig. 6b,c). Data are mean ± s.d.