Fig. 2. Engineering and optimization of the strictosidine platform strain.
a, Strictosidine production by strains MIA-AU and MIA-AW-2 grown in SC medium supplemented with strictosidine pathway precursor geraniol. b, Strictosidine production by engineered strains grown in SC medium supplemented with different intermediates of the strictosidine pathway. c,d, De novo strictosidine production from strains grown in SC (c) and YPD (d) medium. Data are presented as mean ± s.d. (n = 3–4) (a,c,d). *P value 0.05; **P < 0.01. Student’s two-tailed t-test. More statistical analysis is available in the source data file.