Fig. 3. Functionalization of strictosidine-β-d-glucosidase (SGD) in yeast.
a, THA production in yeast strains expressing CroSTR and CroTHAS together with SGD from C. roseus (CroSGD) or R. serpentina (RseSGD). conc., concentration. b, RseSGD protein divided into four domains on the basis of sequence conservation between CroSGD and RseSGD, denoted as R1 (yellow), R2 (blue), R3 (red) and R4 (cyan); crystal structure (PDB ID 2jf6). c, THA production from hybrid SGDs constructed by shuffling four domains between C. roseus (indicated by C) and R. serpentina (indicated by R) sequences. The first letter of the hybrid SGDs on the x axis is domain 1, the second letter domain 2 and so on. Data are presented as mean ± s.d. (n = 3) (a,c). *P < 0.01; **P <0.0001. Student’s two-tailed t-test. More statistical analysis is available in the source data file.