Acupuncture reduced mRNA and protein expressions of P2X4, OX42, BDNF and IRF-5 in IBS-D rats. A. Relative mRNA levels of OX42, P2X4, IRF-5 and BDNF determined by qRT-PCR. B. Protein levels of OX42, BDNF, P2X4 and IRF-5 determined by western blot. ***P < 0.001; **P < 0.01.