Table 4.

Hydrogen bonds between residue in position 343 and Glu333 and trimannose of WT SP-D•Trimannose, Asp325Ala SP-D•Trimannose, Arg343Val SP-D•Trimannose, and DM SP-D•Trimannose at the medial trimannose binding pose.

System	Hbond Type	Acceptor	Donor	Occupancy	Distance (Å)	Angle (degrees)
WT SP-D●Trimannose	Glu333-Arg343	Glu333@OE1	Arg343@NE	0.13	2.84	157.52
		Glu333@OE2	Arg343@NE	0.12	2.84	156.02
		Glu333@OE1	Arg343@NH1	0.25	2.80	158.00
		Glu333@OE1	Arg343@NH2	0.04	2.82	156.05
		Glu333@OE2	Arg343@NH1	0.27	2.80	157.57
		Glu333@OE2	Arg343@NH2	0.05	2.81	156.04
		Total		0.87
	Glu333-Trimannose	Glu333@OE1	Man3@O2	0.05	2.63	162.02
		Glu333@OE2	Man3@O2	0.05	2.64	162.18
		Glu333@OE1	Man3@O3	0.15	2.67	163.82
		Glu333@OE2	Man3@O3	0.18	2.67	163.63
		Total		0.43
Asp325Ala SP-D●Trimannose	Glu333-Arg343	Glu333@OE1	Arg343@NH1	0.16	2.81	156.57
		Glu333@OE2	Arg343@NH1	0.12	2.81	156.60
		Glu333@OE1	Arg343@NH2	0.11	2.82	151.39
		Glu333@OE2	Arg343@NH2	0.08	2.81	150.55
		Glu333@OE1	Arg343@NE	0.05	2.83	153.50
		Glu333@OE2	Arg343@NE	0.19	2.83	154.53
		total		0.70
	Glu333-Trimannose	Glu333@OE1	Man3@O2	0.19	2.65	162.57
		Glu333@OE2	Man3@O2	0.15	2.68	161.59
		Glu333@OE1	Man3@O3	0.09	2.70	163.67
		Glu333@OE2	Man3@O3	0.05	2.71	163.73
		total		0.49
Arg343Val SP-D●Trimannose	Glu333-Val343	No Hydrogen bonds
	Glu333-Trimannose	Glu333@OE1	Man3@O2	0.04	2.71	156.56
		Glu333@OE2	Man3@O2	0.04	2.72	156.49
		Glu333@OE1	Man3@O3	0.48	2.67	163.77
		Glu333@OE2	Man3@O3	0.43	2.67	163.68
		total		1.00
DM SP-D●Trimannose	Glu333-Val343	No Hydrogen bonds
	Glu333-Trimannose	Glu333@OE1	Man3@O2	0.23	2.72	157.01
		Glu333@OE2	Man3@O2	0.20	2.73	156.31
		Glu333@OE1	Man3@O3	0.41	2.68	164.30
		Glu333@OE2	Man3@O3	0.35	2.68	164.24
		total		1.19