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Substrate specificity of EstRag on p-nitrophenyl esters
| p-NP esters | Specific activity (U/mg) | Relative activity (%) | Km (mM) | Kcat (sec−1) | Kcat/Km (sec−1 mM−1) |
|---|---|---|---|---|---|
| p-NP-C2 | 0.470 ± 0.0002 | 100.00 | 0.031 | 20.39 | 657.7 |
| p-NP-C4 | 0.110 ± 0.0008 | 25.77 ± 0.02 | ND | ND | ND |
| p-NP-C6 | 0.089 ± 0.0003 | 18.99 ± 0.05 | ND | ND | ND |
| p-NP-C8 | 0.000 | 0.00 | ND | ND | ND |
| p-NP-C12 | 0.000 | 0.00 | ND | ND | ND |
ND not detectable
Values are the average of three readings ± standard error (SE)
