. 2022 Sep 7;38(12):217. doi: 10.1007/s11274-022-03402-5

Table 5.

Comparison between different reported cold-adapted esterases concerning biochemical properties

Microbial source	Enzyme designation	Expression host	Amino acid length	Optimal pH	Optimal temperature (^oC)	Metal ion resistance	Detergent resistance	Molecular weight (kDa)	Family	Reference
Lysinibacillus sp.	EstRag	E. coli BL21 (DE3) Rosetta	217	8.0	35	Significantly augmented 3 times fold its normal activity in the presence of copper ions	Strongly enhanced by SDS Resistant to triton X-100	25	New member of family VI	This study
Psychrobacter pacificensis	Est11	E. coli BL21 (DE3)	297	7.5	25	Inhibited by Cu²⁺ and Zn²⁺ Resistant to Mg²⁺, Ca²⁺ and Mn²⁺	Resistant to tween 20, tween 80 and triton X-100	32.9	New family	Wu et al. (2015)
Pseudomonas mandelii	EstK	E. coli		8.5	40	Inhibited by Cu²⁺ Resistant to Ca²⁺, K⁺ and Mg²⁺	Not tested	33	Not mentioned	Hong et al. (2012)
Bacillus halodurans	estHIJ	E. coli BL21 (DE3) Rosetta	248	7–8	30	Na⁺, K⁺, Mg²⁺, and Ca²⁺ resistant	Inhibited by SDS Resistant to tween 20, tween 80 and triton X-100	29	XIII	Noby et al. (2019)
Bacillus licheniformis	Est700	E. coli BL21 (DE3)	208	8.0	30	Resistant to Mg²⁺, Ca.²⁺ Ba²⁺, Mn²⁺, Na⁺ and K⁺	Inhibited by SDS Resistant to tween 20, tween 80 and triton X-100	25	I	Zhang et al. (2018)
Bacillus cohnii strain N1	EstN7	E. coli BL21 (DE3) Rosetta	320	9.0	5	Inhibited by Cu²⁺ and Zn²⁺ Resistant to Mn²⁺, K⁺, Na⁺ and Ca²⁺	Inhibited by SDS Resistant to tween 20, tween 80 and triton X-100	37	IV	Noby et al. (2018)
Salinisphaera sp. P7-4	P7-4 esterase	E. coli BL21 (DE3)	316	8.0–9.0	25	Inhibited by Cu,²⁺ Ni²⁺ and Zn²⁺ Resistant to Ca²⁺, K⁺ and Mg²⁺	Not tested	34.4	Not mentioned	Kim et al. (2011)
Psychrobacter cryohalolentis K5T	EstPc	E. coli BL21 (DE3)	315	8.5	35	Inhibited by Cu²⁺ and Zn²⁺ Resistant to Mg²⁺, Mn²⁺ and Co²⁺	Inhibited by SDS Resistant to tween 20,and triton X-100	33	V	Novototskaya-Vlasova et al. (2012)
Pseudoalteromonas sp. strain 643A	EstA	E. coli TOP10F’	207	8.0	35	Inhibited Zn²⁺, Mg²⁺, Co²⁺ and Cu²⁺ Strongly activated by Ca²⁺	Not tested	23	GDSL family of lipolytic enzymes	Cieśliński et al. (2007)
Pseudoalteromonas arctica	EstO	E. coli TunerTM (DE3)	400	7.5	25	Completely inhibited by Al²⁺, Cu²⁺, Fe²⁺, Cr²⁺, and Co2, whereas Ca²⁺, Mg²⁺, Se²⁺ and Mn²⁺ had no or only minor effect	Completely inhibited by SDS and Tween 20	44.1	Serine hydrolase family	Khudary et al. (2010)
Deinococcus radiodurans	EstDR4	E. coli	312	8.0	30	Resistant to Li⁺, Na⁺, K⁺, and Mg²⁺ significantly inhibited by Co²⁺, Cu²⁺ and Zn²⁺	Significantly activated by Tween 80 and Triton X-100 inhibited by SDS and Tween 20	33	IV	Zhang et al. (2021)
Streptomyces coelicolor A3(2)	EstC	E. coli BL21 (DE3)	327	8.5–9.0	35	Significantly inhibited by Cu²⁺, Zn²⁺, Ni²⁺, Fe²⁺ and Mn²⁺ Resistant to Ca ²⁺ and Mg²⁺	Not tested	35	v	Brault et al. (2012)
Acinetobacter venetians V28	V28 esterase	E. coli BL21 (DE3)	338	9.0	40	No significant inhibition of activity was obtained with Ca²⁺, Cu²⁺, Co²⁺, Cd²⁺, Mg²⁺, K⁺, Mn²⁺ and Zn²⁺	Resistant to Tween 20. Tween 80 and SDS	35	Not mentioned	Kim et al. (2012)
Microbulbifer thermotolerans	MtEst45	E. coli BL21 (DE3)	495	8.17	46.2	Strongly inhibited by Hg²⁺, Zn²⁺, and Cu²⁺	Not tested	45.5	III	Lee (2016)
Microbulbifer thermotolerans DAU221	CEST	E. coli	307	8.0	15	Enzyme activity was increased by Na⁺and Mg²⁺ ions but was strongly inhibited by Cu²⁺ and Hg²⁺ ions	Not tested	31.24	VI	Lee et al. (2014)
Metagenomic library	Est97	E. coli	247	7.5	35	Strongly inhibited by Zn²⁺, and Cu²⁺ Resistant to Ca²⁺ and Mg²⁺	Inhibited by SDS and Tween 20	26.9	VIII	Fu et al. (2013)