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. Author manuscript; available in PMC: 2023 Sep 6.
Published in final edited form as: Circulation. 2022 Aug 8;146(10):770–787. doi: 10.1161/CIRCULATIONAHA.121.057620

Fig. 1: GATA4 protein interacts with RNA splicing proteins in cardiac progenitors.

Fig. 1:

(A) Schematic of the experimental approach to identify human GATA4-interacting proteins in iPSC-derived cardiac progenitor cells (CPCs) by AP-MS using antibody to endogenous GATA4. Proteins isolated in an iPSC line lacking GATA4 were subtracted. (B) RNA binding proteins that were isolated with GATA4 from human iPS-CPs. The interaction network was realized using String. (C) Western blot (WB) of splicing factors indicated after Flag immunoprecipitation (IP) using anti-Flag antibody in HEK293T cells expressing Flag-GATA4. Protein expression in input lysate indicated. Cell lysate were treated with RNase and DNase before IP.