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. Author manuscript; available in PMC: 2023 Sep 6.
Published in final edited form as: Circulation. 2022 Aug 8;146(10):770–787. doi: 10.1161/CIRCULATIONAHA.121.057620

Table 5.

In vitro splicing templates

Minigene Name Sequence
SLX4IP-minigene GTCTGTAGTTACTGTGGAATCAATAAGCCATGGCATCTAAGA
AATTTGCTGTTAAAgtaagtaatgtttaatagctttttaaaaagaggctaatcactttctaatat
aactgagctctgtgttaattgaaaatcgaggttcttatgtagaggcaaagcccatgccctcaggccttcc
gatctctcttctctgtgcatgtgcatgcacactcacatgtccccgctcacaaacccactcactttcaacct
cacaggacttcttcgaagatgccagcctccttttcacagcctttgtgtttactactcctgctgcctacaatg
ctcttcccccagatattgctgggcatgagccgcacagacactgactctgccattaatgtctttcagTG
TGGGAATTTTGCTGTCCTCGTGGATCTTCATATCTTGCCACAA
GGTTCAAACAAAGATACAAGCTGGTTTTCTGAACAGAAGAAA
GAGgtacaacaaaactttctactatttaattgcaagtagctgctgctgctataagaaatatcagctctttc
cagctttcatttctgttagtgggaaaaaaatgttgcgtatttaatccttgaaaatttcccagatcacacagtg
agccaacatcatggtttcgagcaaacttcagtagcaaatttaaatttgcggtgttattcatgagcacatatg
acattcctttcaacattcacagctaaattaaaaagcaaaatgaaatgaaatgttcattgttaactttttaatat
atatgttaatttttttgcattaaatttgaaggttatatctaattttttatttgctttagGAAGTCTGTTT
ACTGTTAAAAGAAACCATTGATTCAAGAGTTCAGGAGTACTT
GGAAGTTCGCAAACAGCACAGGCCATCAAATGCAGAATTCAC
AAGATCCAATCCCTTGTCCTTAAAAG
SLX4IP-Motif-Mutant-minigene GTCTGTAGTTACTGTGGAATCAATAAGCCATGGCATCTAAGA
AATTTGCTGTTAAAgtaagtaatgtttaatagctttttaaaaagaggctaatcactttctaatat
aactgagctctgtgttaattgaaaatcgaggttcttatgtagaggcaaagcccatgccctcaggcatca
gtagaacttccgatctgactcttctctgtgcatgtgcatgcacactcacatgtccccgctcacaaaccca
ctcactttcaacctcacaggacttcttcgaagatgccagcctccttttcacagcctttgtgtttactactcct
gctgcctacaaatcagtagcccccagatattgctgggcatgagccgcacagacactgactctgccatt
aatgtctttcagTGTGGGAATTTTGCTGTCCTCGTGGATCTTCATATC
TTGCCACAAGGTTCAAACAAAGATACAAGCTGGTTTTCTGAA
CAGAAGAAAGAGgtacaacaaaactttctactatttaattgcaagtagctgctgctgctataa
gaaatatcagctctttccagctttcatttctgttagtgggaaaaaaatgttgcgtatttaatccttgaaaattt
cccagatcacacagtgagccaacatcatggtttcgagcaaacttcagtagcaaatttaaatttgcggtgt
tattcatgagcacatatgacattcctttcaacattcacagctaaattaaaaagcaaaatgaaatgaaatgtt
cattgttaactttttaatatatatgttaatttttttgcattaaatttgaaggttatatctaattttttatttgctttagG
AAGTCTGTTTACTGTTAAAAGAAACCATTGATTCAAGAGTTC
AGGAGTACTTGGAAGTTCGCAAACAGCACAGGCCATCAAATG
CAGAATTCACAAGATCCAATCCCTTGTCCTTAAAAG
GATA4-minigene ACAACGTCTCCCTCCCACCCGGCTGAGAACAGCCTGGAATCC
CTGTGCAGAGTTTGCCTCACACCAGGCCTTGAGCAGCCGCAG
GGACGCCGCTCCCCACCGCGGgtgagtcccctcctggccctcctggtgacccctct
gccgtgggaggctgggatgctgggggaatggaaggcttggggagtctccttgctcaccacaatccg
gaggctctacgtccactccgtatccccaagaaagagtgtccgaggcacggaccatagcaagtgaag
gaaggtaggtcgacgtggccttgcagctgaattcgttctccatttttccttcagcagGGACGCAT
CCTGCTCTGCACCCTGGTTCTCGGCGCTGCGCCCGCGGAGGCT
CGTGCAGGGCAGGCTGCCCGTGCGGGTGAGGACTGAGTGCCG
CGCAGGGAAGGAGTATCGCAGACCGGCGCCCAGGCCCAGCG
GGGGAATCCAAGGGCCGTGTTGCAGGACTCGGCATTCGTTCT
GCGCGGGTCACCTTGAATGTCTGTCCGGATCCCTCGCGGCAG
GGCCGCAGAGGCGCGTCCATATCTTGGAGGAATTCGTTCCAT
AGAATGAGgtttgattctctctggggtttcttgttttccattataagactctggcgaccttggtggcg
ccagattttttcagatgttgcttttgttccgggtgtagcgcccggctgcggcgaggaggaaggagccag
cctagcagcttctgcgcctgtggccgcgggtgtcctggaggcctctcggtgtgacgagtgggggacc
cgaaggctcgtgcgccacctccaggcctggacgctgccctccgtcttctgcccccaataggtgcgcc
ggaccttcaggccctggggtgaattcagctgctcctacatcagcttccggaaccaccaaaaattcaaat
tgggattttccggagtaaacaagagcctagagccctttgctcaatgctggatttaatacgtatatatttttaa
gcgagttggttttttcccctttgatttttgatcttcgcgacagttcctcccacgcatattatcgttgttgccgtc
gttttctctccccgcgtggctccttgacctgcgagggagagagaggacaccgaagccgggagctcgc
agggaccatgtatcagagcttggccatggccgccaaccacgggccgccccccggtgcctacgagg
cgggcggccccggcgccttcatgcacggcgcgggcgccgcgtcctcgccagtctacgtgcccaca
ccgcgggtgccctcctccgtgctgggcctgtcctacctccagggcggaggcgcgggctctgcgtcc
ggaggcgcctcgggcggcagctccggtggggccgcgtctggtgcggggcccgggacccagcag
ggcagcccgggatggagccaggcgggagccgacggagccgcttacaccccgccgccggtgtcgc
cgcgcttctccttcccggggaccaccgggtccctggcggccgccgccgccgctgccgcggcccgg
gaagctgcggcctacagcagtggcggcggagcggcgggtgcgggcctggcgggccgcgagcag
tacgggcgcgccggcttcgcgggctcctactccagcccctacccggcttacatggccgacgtgggc
gcgtcctgggccgcagccgccgccgcctccgccggccccttcgacagcccggtcctgcacagcctg
cccggccgggccaacccggccgcccgacaccccaatctcggtgagtaggagcgcgagggctggg
gcgcgtgagggccggggcaggggccgtcttgagccctgtcgagggcctcttgtttttccaccaacgc
cttcgttgggctggggatggtgcttcactacctcgagtttctagggaaggcagaagccagtgcggggc
tggcgacatcacagccccagaagaccggcttctgtggaaggggccgggcctgcccgccggggcct
cttctgagatggtgtcagggtcggagtgaggtggtcttctctttccaaggaaagggcattgtttctgtgcg
ctctagattctcagatgtgagagctgggcataaacaaagaattaatcctctgtgtcttttcttgtctgttccc
cccaactcagTAGATATGTTTGACGACTTCTCAGAAGGCAGAGAGT
GTGTCAACTGTGGGGCTATGTCCACCCCGCTCTGGAGGCGAG
ATGGGACGGGTCACTATCTGTGCAACGCCTGCGGCCTCTACC
ACAAGATGAACGGCATCAACCGGCCGCTCATCAAGC
TTYH3-minigene GCCCTGCTGCTCCTGGGGGCCGCCGCCCTGGCCTGCCTCGCCC
TGGACCTCCTCTTCCTGCTCTTCTACTCCTTCTGGCTGTGCTGC
CGGCGGCGCAAGAGCGAGGAGCACCTGGACGCCGACTGCTG
CTGCACGGCCTGGTGTGTCATCATCGCCACGCTGGTGTGCAGg
tgagcgcggtggggcggggacggagggcggggccagagggggcggcccgagggggcggggc
tggagtggggtgtgtgtgggtgggcggggctacatctcacgggcccgccctctccagCGCCGG
CATCGCAGTGGGATTCTACGGCAACGGGGAGACCAGTGATGG
CATCCATAGGGCCACCTACTCGCTCCGCCACGCCAACCGCAC
GGTGGCCGGGGTCCAGGACCGCgtgagtggccgcggagttggggccaggagc
actcttgctgctccggagccccacgtctgcgcgaggacgggcggggcagccgggactggggctgt
gcaggaggggcccagactctggggcgaggcgggcgcgctcccagcctcacgcccgcgggtccg
gcgcagGTGTGGGACACGGCGGTGGGGCTGAACCACACGGCGG
AGCCCAGCCTGCAGACCCTGGAGCGGCAGCTGGCCGGGCGG
CCCGAGCCCCTGCGAGCCGTACAGAGGCTGCAGGGCCTGCTG
GAGACGCTGCTGGGCTACACGGCCGCCATCCCCTTTTGGAGG
AACACGGCGGTGTCGCTGGAGGTGCTGGCGGAGCAGGTGGA
TCTCTACGACTGGTACAG