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. Author manuscript; available in PMC: 2022 Sep 8.
Published in final edited form as: Ecotoxicol Environ Saf. 2022 Jul 5;241:113829. doi: 10.1016/j.ecoenv.2022.113829

Fig. 6.

Fig. 6.

Pb-induced hippocampal neurons apoptosis though IP3R-Ca2+-ASK1-p38 signaling pathway. a Primary hippocampal neurons were treated with increased concentrations of PbAc for 24 h. The activations of ASK1, JNK and p38 were determined by western blotting. b, c Primary hippocampal neurons were treated with 50 μM PbAc in the presence or absence of pretreatment with 2-APB or BAPTA-AM for 24 h. The activations of ASK1 and p38 were determined by western blotting. d, e Primary hippocampal neurons were treated with 50 μM PbAc in the presence or absence of pretreatment with SB203580 for 24 h. d Hippocampal neuron apoptosis was determined by flow cytometry analysis. e Protein level of cleaved caspase-3, Bax and Bcl-xL were determined using western blotting. The protein expression was normalized by GAPDH or corresponding total protein content. * P < 0.05 and * * P < 0.01, compared to the control group. #P < 0.05 and ##P < 0.01, compared to the Pb-treated group.