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. 2022 Aug 25;10:965595. doi: 10.3389/fcell.2022.965595

FIGURE 1.

FIGURE 1

Decreasing RB protein concentration in mouse liver reduces hepatocyte size. (A). Schematic of experimental timeline performed using Rosa-rtTA; TRE-shRb1 mice. (B). Immunoblot and qPCR analysis following shRb1 induction in mouse liver (n = 4). Mice were harvested after 4 weeks of Dox treatment (1 g/L) that started at birth. (C). Coulter counter cell size measurement of the hepatocytes isolated from Rosa-rtTA (Ctrl, n = 4) and Rosa-rtTA; TRE-shRb1 (shRb1, n = 4) mice at 3 weeks after Dox removal. (D) Schematic of experimental timeline performed using Rb1 flx/flx ; Alb-CreERT2 mice. (E) Immunoblot shows RB protein concentrations in the livers of Rb1 +/+ , Rb1 +/- , and Rb1 −/− mice. Mice were harvested at 6 weeks after Tamoxifen treatment. The right panel shows the quantification of protein concentration. RB protein concentration was normalized using actin. (F) Cell size measurements were made using a Coulter counter to examine hepatocytes isolated from Rb1 +/+ (n = 6 for tetraploid, n = 4 for octoploid), Rb1 +/− (n = 8 for tetraploid, n = 4 for octoploid), and Rb1 −/− (n = 6 for tetraploid, n = 4 for octoploid) mice at 6 weeks after Tamoxifen treatment. Each dot represents data from one mouse. The error bars indicate standard deviation. **p < 0.01.