FIGURE 2.

Increasing Rb1 concentration in mouse liver increases hepatocyte cell size. (A) Schematic of Fah−/− system as a platform for genetic manipulation of hepatocytes in the liver. (B) Schematic of experimental timeline. Fah ^−/− mice were injected with Fah transposons as indicated, and their livers were harvested 4 weeks after the injection. (C,D) Immunofluorescence staining (C) and immunoblots (D) were performed to measure Fah and RB expression. (E) Cell size measurement of the hepatocytes isolated from Fah ^−/− mice injected with Fah transposons were made with a Coulter counter. Each dot represents data from one mouse (n = 8 for tetraploid, n = 7 for octoploid). The error bars indicate standard deviation. *p < 0.05.