TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Genotype or relevant characteristic(s) | Reference or source |
|---|---|---|
| Strains | ||
| E. coli | ||
| DH5α | F−recA1 endA1 gyrA96 thi-1 hsdR17(rK− mK+) sup44 relA1 λ− φ80dlacZΔM15(lacZYA-argF)U169 | Gibco BRL |
| DH5αMCR | F−mcrA Δ(mrr-hsdRMS-mcrBC) φ80dlacZΔM15 Δ(lacZYA-argF)U169 deoR recA1 endA1 supE44 λ− thi-1 gyrA96 relA1 | 14 |
| RDE51 | araD139 thi Δ(lac)U168 strA dapE::MuCts | 25 |
| AT982 | rel-1 thi-1 dapD4 | 7 |
| AT997 | rel-1 thi-1 dapC15 | 7 |
| SM10 | RP4-2-Tc::Mu thi thr leu suIII Kanr; mobilizing donor strain | 31 |
| B. pertussis | ||
| Tohama I (TI) | Wild type | Rino Rappuoli |
| TIΔdapC | Allelic exchange mutant of TI with a 228-bp deletion in dapC | This study |
| Plasmids | ||
| pBluescript SK | High-copy cloning vector | Stratagene |
| pSS1129 | Bordetella suicide vector | 34 |
| pSK50 | 5.0-kb EcoRI fragment from TI in pSK including the dap operon of B. pertussis (Fig. 2) | This study |
| pSK-dapC | 2.9-kb ApaI fragment from pSK50 containing dapC and truncated dapD (Fig. 2) | This study |
| pSK-ΔdapC | Derivative of pSK-dapC with a 228-bp NcoI deletion | This study |
| pSS-ΔdapC | EcoRI-BamHI fragment from pSK-ΔdapC joined to pSS1129 | This study |