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IL-22 inhibited the expression of proinflammatory cytokines in DSS-induced colitis mice colons. qRT-PCR was performed to determine proinflammatory cytokine mRNA expression levels (TNF-α, IL-6, IL-1β, IFN-γ, IL-18, IL-17A, CXCL1, and CCL2) in colon tissue. (b) ELISA assay was used to detect levels of soluble TNF-α, IL-6, IL-1β, and IL-17 in the medium of the colon tissues. Data are presented as mean ± SEM. ∗∗∗P < 0.001; ∗∗P < 0.01; ∗P < 0.05 using Student's t-test.
