Figure 6.

Claudin-1 is the direct target of miR-568 in intestinal epithelial cells. Sequence alignment showing the binding sites between miR-568 and the 3′-UTR of claudin-1 mRNA. (b) Western blot analysis was used to detect changes in claudin-1 protein expression under the effect of miR-568 mimics and inhibitor in MODE-K cells. (c) Luciferase assay of HEK-293T cells cotransfected with miR-568 mimics and wild-type or mutant 3′-UTR of the claudin-1 mRNA sequence. (d–f) qRT-PCR, western blotting, and immunohistochemistry analyses were conducted to determine claudin-1 expression levels in the colon tissues of all groups of mice. Data are presented as mean ± SEM. ^##P < 0.01; ^#P < 0.05 using Student's t-test.