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Non-synonymousamino acid substitutions shared in each sub-lineage of G2 lineage IV where South African P[4] strains in this study clustered in respect to the outgroup P[4] strain, RVA/Human-tc/USA/DS-1/1976/G2P[4], are shown. South African pre-vaccine G2 sequences are indicated with black circles while post-vaccine strains are highlighted in red circles. Nodes with defining non-synonymous amino acid substitutions are highlighted in blue rectangles.
