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. 2022 Aug 31;2022:9916228. doi: 10.1155/2022/9916228

Figure 2.

Figure 2

The effect of exosomal PCAT1 on the biological behavior of circulating tumor T84 cells. (a) RT-qPCR was used to detect the expressions of miR-329-3p after co-culture of exosomes with T84. (b) Western blot was used to detect the expressions of Netrin-1 and CD146 after co-culture of exosomes with T84. (c) CCK-8 detected the cell proliferation activity after co-culture of exosomes with T84. (d) Transwell detected the cell migration ability after co-culture of exosomes with T84. ∗∗P < 0.01, ∗∗∗∗P < 0.0001vs Control. (e) RT-qPCR. was used to detect the expressions of miR-329-3p in exosomes co-cultured with T84 after PCAT1 knockdown. (f) Western blot was used to detect the expressions of Netrin-1 and CD146 in exosomes co-cultured with T84 after PCAT1 knockdown. (g) CCK-8 detected the cell proliferation activity of exosomes and T84 co-cultured after PCAT1 knockdown. (h) Transwell detected the cell migration ability of exosomes and T84 co-cultured after PCAT1 knockdown. ∗∗P < 0.01, ∗∗∗∗P < 0.0001vs co-EXO+sh-NC.