Figure 3.

The effect of exosomal PCAT1 on the EMT process and fibrous actin. (a) Western blot was used to detect the expression of EMT-related proteins in exosomes co-cultured with T84. (b) The changes of F-actin after co-culture of exosomes with HUVECs were detected by immunofluorescence. ^∗∗P < 0.01vs Control. (c) Western blot was used to detect the expression of EMT-related proteins in exosomes co-cultured with T84 after PCAT1 knockdown. (d) The changes of F-actin in exosomes and HUVECs co-cultured after PCAT1 knockdown were detected by immunofluorescence. ^∗∗P < 0.01vs co-EXO+sh-NC.