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. 2022 Aug 25;15:769725. doi: 10.3389/fnmol.2022.769725

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Copyright © 2022 Priel, Dai, Chen, Scarinci, Cantero and Cantiello.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Isolation and imaging of adult mouse hippocampus dendritic spines. (A) Isolation procedure of DS developed in the present study. Adapted from Kiebler et al. (1999). (B) Isolated DS could be identified by DIC (x100) (panels 1 and 2 from Top to Bottom, vertical line 5 μm), and recognized as “wrinkled sac” shape, of approximately 1 μm diameter, as compared with 1 μm beads (Bottom panel). (C, Left) TRITC-phalloidin labeling of fresh (not-fixed) DS allowed rapid identification of abundant F-actin inside DS under fluorescence. (Right) 1 μm diameter fluorescent beads are shown for size comparison.