TABLE 2.
Sequence, product size and accession number of primers for quantitative real-time PCR.
| Item | Nucleotide sequence of primers (5′→3′) | Product size (bp) | GenBank accession |
| Claudin-1 | F: CATACTCCTGGGTCTGGTTGGT | 100 | NM 001013611.2 |
| R: GACAGCCATCCGCATCTTCT | |||
| Claudin-5 | F: CATCACTTCTCCTTCGTCAGC | 111 | NM_204201 |
| R: GCACAAAGATCTCCCAGGTC | |||
| Occludin | F: ACGGCAGCACCTACCTCAA | 123 | XM 025144247.2 |
| R: GGGCGAAGAAGCAGATGAG | |||
| ZO-1 | F: CTTCAGGTGTTTCTCTTCCTCCTC | 131 | XM 030016322.2 |
| R: CTGTGGTTTCATGGCTGGATC | |||
| IL-1β | F: ACTGGGCATCAAGGGCTACA | 142 | Y15006.1 |
| R: GCTGTCCAGGCGGTAGAAGA | |||
| IL-6 | F: CTCCTCGCCAATCTGAAGTC | 99 | NM_204628 |
| R: CCTCACGGTCTTCTCCATAAAC | |||
| IL-8 | F: GGCTTGCTAGGGGAAATGA | 200 | DQ393272.2 |
| R: AGCTGACTCTGACTAGGAAACTGT | |||
| IL-17 | F: CTCCTCTGTTCAGACCACTGC | 126 | AJ493595.1 |
| R: ATCCAGCATCTGCTTTCTTGA | |||
| TGF-β | F: AGGATCTGCAGTGGAAGTGGAT | 138 | M31160 |
| R: CCCCGGGTTGTGTTGGT | |||
| TNF-α | F: GAGCGTTGACTTGGCTGTC | 64 | GU230788.1 |
| R: AAGCAACAACCAGCTATGCAC | |||
| β-actin | F: ATGATATTGCTGCGCTCGTT | 145 | L08165.1 |
| R: TCTTTCTGGCCCATACCAACC |
ZO-1, zonula occludens-1; IL-1β, interleukin-1β; IL-6, interleukin-6; IL-8, interleukin-8; IL-17, interleukin-17; TGF-β, transforming growth factor-β; TNF-α, tumor necrosis factor-α.