Table 3.
Bone marrow cotransplantation model
|
Ref.
|
HSC donor
|
MSC donor
|
Host
|
Conditioning
|
Source
|
Dose (Gy)
|
MSC immunogenicity
|
Groups
|
| Lee et al[9] | Mice (C57BL/6) | Human | Mice (BALB/c H-2d) | Chemotherapy | Bu/Cy | 25 mg/kg/4 d | Xenogeneic | Control; HSC; HSC + T-MSC; HSC + MMP3kdT-MSC |
| 100 mg/kg/2 d | ||||||||
| Huang et al[10] | Human | Human | Mice (NOD/SCID) | TBI | 60-Co | 2.7 | Xenogeneic | Control; HSC; HSC + UCB-MSC (IV/IB) |
| Yin et al[8] | Human | Human | Mice (NOD/SCID; NOG) | TBI | NR | 2.5 | Xenogeneic | HSC; HSC + GFP-MSC; HSC + EGF-MSC; HSC + FGF2-MSC; HSC + PDGFB-MSC (IV/IB) |
| Choi et al[11] | Mice (C57BL/6) | Human | Mice (BALB/c) | Chemotherapy | Bu/Cy | 20 mg/kg/4 d | Xenogeneic | Control; HSC; HSC + T-MSC |
| 100 mg/kg/2 d | ||||||||
| Trento et al[12] | Mice (C57BL/6 CD45.1) | Mice (C57BL/6 CD45.2 orNos2-/-) | Mice (C57BL/6 CD45.2) | TBI | NR | 8 | Allogeneic | HSC; HSC + MSC; HSC + Nos2−/−MSC |
| Abbuehl et al[13] | Mice (FVB Actin-GFP) | Mice (FVB Insulin-GFP) | Mice (FVB Insulin-GFP) | TBI | NR | 2 × 4.5 | Allogeneic | HSC; HSC + MSC |
| Kim et al[14] | Mice (C57BL/6J-Pep3b-Ly5.1 Pep3b) | Mice (C57BL/6J-Ly 5.2 BL6) | Mice (C57BL/6J-Ly 5.2 BL6) | TBI | NR | 9 | Xenogeneic | HSC; HSC + MSC (NSS/SS) direct or priming |
| Futrega et al[15] | Human | Human | Mice (NSG) | TBI | 137-Cs | 2.5 | Xenogeneic | HSC (IV/IB); HSC + MSC; HSC + MSC-spheroids (IB) |
| van der Garde et al[16] | Human | Human | Mice (NOD/SCID) | TBI | NR | 3.5 | Xenogeneic | HSC; HSC + MSC; Ex/TPO-HSC; Ex/TPO-HSC + MSC |
| Fernández-García et al[17] | Mice (P3D2F1 H2b/d CD45.1) | Mice (B6D2F1,H2b/d, CD45.2) | Mice (B6D2F CD45.2) | TBI | NR | 5-7 | Allogeneic | HSC; HSC + AT-MSC |
| Chen et al[18] | Mice (C57BL/6) | Mice (C57BL/6) | Mice (BALB/c H-2d) | TBI | 60-Co | 7.5 | Allogeneic | HSC; HSC + EGFP-MSC; HSC + CXCR4-MSC |
| Chen et al[19] | Human | Human | Mice (NOD/SCID/IL2rγnull) | TBI | 60-Co | 3.5 | Xenogeneic | Control; HSC; HSC + MSC; HSC + SDF-1-MSC; HSC + HOXB4-MSC; HSC + SDF1-HOXB4-MSC |
| Wu et al[20] | Human | Human | Mice (NOD/SCID) | TBI | NR | 3.5 | Xenogeneic | HSC; HSC + UCB-MSC |
| Lim et al[21] | Human | Human | Mice (NOD/SCID) | TBI | Cs | 2.5 | Xenogeneic | HSC; HSC + hPTH; HSC + UCB-MSC + hPTH |
| Lee et al[22] | Human | Human | Mice (NOD/SCID) | TBI | NR | 3 | Xenogeneic | HSC; HSC + BM-MSC; HSC + AT-MSC; HSC + UCB-MSC |
| Kornblit et al[23] | Dog (beagle) | Dog (beagle) | Dog (Beagle) | TBI | 60-Co | 9.2 | Allogeneic | Control; HSC; HSC + MSC (unrelated or DLA-id MSC) |
| Fortin et al[24] | Mice (B6.SJL-PtrcaPep3b/BoyJ CD45.1) | Mice | Mice (C57BL/6J) | TBI | 60-Co | 8 | Allogeneic | HSC; HSC + MSC; HSC + solG-CSFR-MSC |
| Carrancio et al[25] | Human | Human | Mice (NOD/SCID) | TBI | Cs | 3 | Xenogeneic | HSC (IV/IB); HSC IV + MSC (IV/IB) |
Bu: Busulfan; Cy: Cyclophosphamide; TBI: Total body irradiation; Cs: Cesium; Co: Cobalt; HSC: Hematopoietic stem cells; Ex/TPO-HSC: HSC expanded with thrombopoietin; MSC: Mesenchymal stem cells; T-MSC: Tonsil mesenchymal stem cells derived; UCB-MSC: Umbilical cord blood mesenchymal stem cells derived; AT-MSC: Adipose tissue mesenchymal stem cells derived; BM-MSC: Bone marrow mesenchymal stem cells derived; MMP3kdT-MSC: Mesenchymal stem cells with matrix metallopeptidase 3-knockdown; GFP-MSC: Mesenchymal stem cells expressing green fluorescent protein; EGF-MSC: Mesenchymal stem cells expressing epidermic growth factor; FGF2-MSC: Mesenchymal stem cells expressing fibroblast growth factor 2; PDGFB-MSC: Mesenchymal stem cells expressing platelet-derived growth factor subunit B; Nos2-/-MSC: Mesenchymal stem cells deficient in type 2 nitric oxide; EGFP-MSC: Mesenchymal stem cells expressing enhanced green fluorescent protein; CXCR4-MSC: Mesenchymal stem cells expressing C-X-C chemokine receptor type 4; SDF-1-MSC: Mesenchymal stem cells expressing stromal cell-derived factor 1; HOXB4-MSC: Mesenchymal stem cells expressing Homeobox B4; solG-CSFR-MSC: Mesenchymal stem cells expressing soluble granulocyte colony-stimulating factor decoy receptor; hPTH: Human parathyroid hormone; DLA: Dog leukocyte antigen; DLA-id: Dog leukocyte antigen identical; NSS: Non-stimulatory serum; SS: Stimulatory serum.