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. 2022 Aug 25;13:978865. doi: 10.3389/fimmu.2022.978865

Figure 8.

Figure 8

In-vitro validation of essential genes (i.e., DLAT, LIPT1, and LIAS) as potential diagnostic biomarkers in PAAD. (A) Results of RT-qPCR of DLAT, LIPT1, and LIAS in HPDE6-C7, CFPAC-1, PANC-1, SW1990, and AsPC-1 cell lines, respectively. (B) Results of western-blot in triple repletion utilizing the aforementioned cell lines. (C) IFS slides in 100X and 400X magnification demonstrated the expressional abundance of these proteins of interest in HPDE6-C7 and AsPC-1 cell lines. (D) Evaluation of the eligibilities of DLAT, LIPT1, and LIAS serving as diagnostic biomarkers in PAAD.