Table A1. Microbiological characteristics, molecular characterization, and susceptibility testing results for 109 ESBL-producing Enterobacteriaceae isolates.
| Isolate number | Collection mmm-yy | Organism | Location | Specimen type | Disk confirmation test |
Molecular results | Antimicrobial susceptibility test (MIC) | |||
|---|---|---|---|---|---|---|---|---|---|---|
| SHV | TEM | CTXM1 | CZA | C/T | ||||||
| 1 | Nov-12 | Klebsiella pneumoniae ssp pneumoniae | SICU | Sputum | Positive | Negative | Negative | Positive | 0.25 | 0.75 |
| 2 | Nov-12 | Klebsiella pneumoniae ssp pneumoniae | SICU | Urine | Positive | Positive | Positive | Positive | 0.25 | 0.50 |
| 3 | Nov-12 | Klebsiella pneumoniae ssp pneumoniae | SICU | Sputum | Positive | Positive | Positive | Positive | 0.75 | 1.00 |
| 4 | Nov-12 | Escherichia coli | SICU | Wound Swab | Positive | Negative | Negative | Positive | 0.12 | 0.38 |
| 5 | Nov-12 | Serratia marcescens | MICU | Blood | Positive | Negative | Negative | Negative | 0.12 | 0.19 |
| 6 | Nov-12 | Klebsiella pneumoniae ssp pneumoniae | PICU | Endotracheal Tube Secretion | Positive | Positive | Negative | Negative | 0.19 | 0.38 |
| 7 | Nov-12 | Klebsiella pneumoniae ssp pneumoniae | NICU | Peritoneal fluid | Negative +AmpC | Negative | Positive | Positive | 0.19 | 0.25 |
| 8 | Nov-12 | Escherichia coli | MICU | Urine | Negative +AmpC | Negative | Positive | Negative | 0.50 | 2.00 |
| 9 | Nov-12 | Klebsiella pneumoniae ssp pneumoniae | SICU | Blood | Positive | Positive | Negative | Negative | 0.75 | 1.50 |
| 10 | Nov-12 | Klebsiella pneumoniae ssp pneumoniae | PICU | Endotracheal Tube Secretion | Positive | Positive | Positive | Positive | 0.19 | 0.38 |
| 11 | Dec-12 | Klebsiella pneumoniae ssp pneumoniae | SICU | Endotracheal Tube Secretion | Positive | Positive | Positive | Positive | 0.19 | 0.38 |
| 12 | Dec-12 | Klebsiella oxytoca | NICU | Tracheostomy Site Swab | Positive | Negative | Negative | Negative | 0.12 | 0.38 |
| 13 | Dec-12 | Klebsiella pneumoniae ssp pneumoniae | PICU | Urine | Positive | Positive | Positive | Positive | 0.25 | 0.50 |
| 14 | Dec-12 | Escherichia coli | NICU | Conjunctival Swab | Positive | Negative | Negative | Positive | 0.09 | 0.38 |
| 15 | Dec-12 | Enterobacter aerogenes | MICU | Blood | Positive | Positive | Negative | Negative | 0.19 | 0.50 |
| 16 | Dec-12 | Klebsiella pneumoniae ssp pneumoniae | MICU | Endotracheal Tube Secretion | Positive | Positive | Positive | Positive | 0.19 | 0.38 |
| 17 | Jan-13 | Escherichia coli | MICU | Urine | Negative | Negative | Negative | Negative | 0.38 | 0.50 |
| 18 | Jan-13 | Escherichia coli | SICU | Blood | Positive | Negative | Positive | Negative | 0.25 | 0.38 |
| 19 | Jan-13 | Escherichia coli | TICU | Blood | Positive | Negative | Positive | Positive | 0.125 | 0.25 |
| 20 | Jan-13 | Enterobacter cloacae | TICU | Blood | Negative | Negative | Negative | Negative | 0.12 | 0.25 |
| 21 | Jan-13 | Klebsiella pneumoniae ssp pneumoniae | SICU | Sputum | Positive | Positive | Positive | Positive | 0.25 | 0.38 |
| 22 | Jan-13 | Escherichia coli | MICU | Blood | Negative +AmpC | Negative | Positive | Negative | 0.38 | 1.50 |
| 23 | Jan-13 | Proteus penneri | MICU | Blood | Positive | Negative | Negative | Negative | 0.047 | 1.00 |
| 24 | Jan-13 | Escherichia coli | PICU | Urine | Positive | Negative | Negative | Positive | 0.12 | 0.38 |
| 25 | Jan-13 | Klebsiella pneumoniae ssp pneumoniae | MICU | Endotracheal Tube Secretion | Positive | Positive | Negative | Negative | 0.25 | 0.38 |
| 26 | Jan-13 | Klebsiella pneumoniae ssp pneumoniae | TICU | Urine | Positive | Positive | Positive | Positive | 0.25 | 0.75 |
| 27 | Jan-13 | Citrobacter braakii | TICU | Blood | Negative | Negative | Negative | Negative | 0.25 | 0.75 |
| 28 | Jan-13 | Escherichia coli | TICU | Sputum | Positive | Negative | Negative | Positive | 0.94 | 0.38 |
| 29 | Jan-13 | Escherichia coli | PICU | Urine | Positive | Negative | Positive | Positive | 0.12 | 0.38 |
| 30 | Feb-13 | Serratia marcescens | MICU | Sputum | Negative | Negative | Negative | Negative | 0.02 | 0.19 |
| 31 | Feb-13 | Escherichia coli | MICU | Urine | Positive | Negative | Positive | Positive | 0.06 | 0.25 |
| 32 | Feb-13 | Escherichia coli | MICU | Blood | Positive | Negative | Negative | Positive | 0.12 | 0.50 |
| 33 | Feb-13 | Klebsiella pneumoniae ssp pneumoniae | MICU | Tracheal Aspirate | Positive | Positive | Positive | Positive | 0.19 | 0.38 |
| 34 | Feb-13 | Klebsiella pneumoniae ssp pneumoniae | SICU | Urine | Positive | Positive | Negative | Positive | 0.09 | 0.38 |
| 35 | Feb-13 | Escherichia coli | MICU | Blood | Positive | Negative | Negative | Positive | 0.19 | 0.38 |
| 36 | Mar-13 | Escherichia coli | TICU | Ascitic Fluid | Positive | Negative | Negative | Positive | 0.12 | 0.38 |
| 37 | Mar-13 | Klebsiella pneumoniae ssp pneumoniae | TICU | Sputum | Positive | Positive | Negative | Negative | 0.25 | 0.38 |
| 38 | Mar-13 | Klebsiella pneumoniae ssp pneumoniae | SICU | Urine | Positive | Negative | Negative | Positive | 0.38 | 0.38 |
| 39 | Mar-13 | Klebsiella pneumoniae ssp pneumoniae | NICU | Blood | Positive | Positive | Negative | Positive | 0.25 | 0.38 |
| 40 | Mar-13 | Klebsiella pneumoniae ssp pneumoniae | SICU | Blood | Positive | Positive | Negative | Positive | 0.25 | 0.38 |
| 41 | Mar-13 | Escherichia coli | SICU | Peritoneal fluid | Positive + AmpC | Negative | Negative | Positive | 256.00 | 256.00 |
| 42 | Mar-13 | Escherichia coli | SICU | Peritoneal fluid | Negative + AmpC | Negative | Negative | Positive | 0.19 | 0.75 |
| 43 | Mar-13 | Escherichia coli | SICU | Urine | Positive | Positive | Negative | Positive | 0.12 | 0.38 |
| 44 | Mar-13 | Klebsiella pneumoniae ssp pneumoniae | PICU | Urine | Positive | Positive | Positive | Positive | 0.19 | 0.5 |
| 45 | Mar-13 | Klebsiella pneumoniae ssp pneumoniae | MICU | Sputum | Positive | Positive | Positive | Positive | 0.38 | 1.00 |
| 46 | Mar-13 | Klebsiella pneumoniae ssp ozaenae | MICU | Sputum | Positive | Negative | Negative | Negative | 0.09 | 0.25 |
| 47 | Mar-13 | Klebsiella pneumoniae ssp pneumoniae | MICU | Tracheal Aspirate | Positive | Negative | Positive | Positive | 0.12 | 0.25 |
| 48 | Mar-13 | Klebsiella pneumoniae ssp pneumoniae | MICU | Urine | Positive | Positive | Positive | Positive | 0.38 | 1.50 |
| 49 | Mar-13 | Escherichia coli | TICU | Urine | Positive | Negative | Positive | Positive | 0.09 | 0.38 |
| 50 | Apr-13 | Klebsiella pneumoniae ssp pneumoniae | TICU | J VAC Fluid | Positive | Negative | Positive | Positive | 0.19 | 0.5 |
| 51 | Apr-13 | Escherichia coli | TICU | J VAC Fluid | Positive | Negative | Negative | Positive | 0.09 | 0.75 |
| 52 | Apr-13 | Escherichia coli | PICU | Urine | Positive | Negative | Negative | Positive | 0.12 | 0.38 |
| 53 | Apr-13 | Klebsiella pneumoniae ssp pneumoniae | PICU | Endotracheal Tube Secretion | Positive | Positive | Positive | Positive | 0.19 | 0.75 |
| 54 | Apr-13 | Enterobacter aerogenes | MICU | Tracheal Aspirate | Positive | Positive | Positive | Positive | 0.25 | 0.38 |
| 55 | Apr-13 | Klebsiella pneumoniae ssp pneumoniae | SICU | Blood | Positive | Positive | Negative | Negative | 0.75 | 1.00 |
| 56 | Apr-13 | Klebsiella pneumoniae ssp pneumoniae | PICU | Endotracheal Tube Secretion | Positive | Positive | Negative | Negative | 0.25 | 0.38 |
| 57 | Apr-13 | Klebsiella pneumoniae ssp pneumoniae | SICU | Blood | Positive | Positive | Negative | Positive | 0.19 | 0.38 |
| 58 | Apr-13 | Escherichia coli | SICU | Wound Swab | Positive | Negative | Negative | Positive | 0.12 | 0.50 |
| 59 | Apr-13 | Klebsiella pneumoniae ssp pneumoniae | SICU | Sputum | Positive | Positive | Negative | Negative | 0.25 | 0.38 |
| 60 | Apr-13 | Klebsiella pneumoniae ssp pneumoniae | SICU | Blood | Positive | Positive | Negative | Negative | 0.19 | 0.25 |
| 61 | Apr-13 | Klebsiella pneumoniae ssp pneumoniae | NICU | Blood | Positive | Positive | Positive | Positive | 0.19 | 0.38 |
| 62 | Apr-13 | Klebsiella pneumoniae ssp pneumoniae | MICU | Endotracheal Tube Secretion | Positive | Positive | Negative | Negative | 0.25 | 0.25 |
| 63 | May-13 | Citrobacter freundii | TICU | Blood | Negative | Negative | Negative | Negative | 0.06 | 0.38 |
| 64 | May-13 | Escherichia coli | MICU | Urine | Positive | Negative | Negative | Positive | 0.06 | 0.25 |
| 65 | May-13 | Klebsiella pneumoniae ssp pneumoniae | PICU | Tracheostomy Site Swab | Positive | Positive | Positive | Positive | 0.25 | 0.75 |
| 66 | May-13 | Klebsiella pneumoniae ssp pneumoniae | NICU | Endotracheal Tube Secretion | Positive | Positive | Positive | Positive | 0.25 | 0.38 |
| 67 | May-13 | Klebsiella pneumoniae ssp pneumoniae | SICU | Sputum | Positive | Positive | Negative | Negative | 0.19 | 0.38 |
| 68 | May-13 | Klebsiella pneumoniae ssp pneumoniae | TICU | Blood | Positive | Positive | Negative | Positive | 0.19 | 0.75 |
| 69 | May-13 | Klebsiella pneumoniae ssp pneumoniae | MICU | Sputum | Positive | Positive | Negative | Negative | 0.38 | 0.38 |
| 70 | May-13 | Enterobacter cloacae | SICU | Sputum | Positive | Positive | Negative | Negative | 0.19 | 0.19 |
| 71 | Jun-13 | Klebsiella pneumoniae ssp pneumoniae | TICU | Wound Swab | Positive | Positive | Negative | Positive | 0.38 | 0.38 |
| 72 | Jun-13 | Klebsiella pneumoniae ssp pneumoniae | SICU | Blood | Positive | Positive | Negative | Negative | 0.19 | 0.38 |
| 73 | Jun-13 | Klebsiella pneumoniae ssp pneumoniae | SICU | Blood | Positive | Positive | Positive | Positive | 0.19 | 1.00 |
| 74 | Jun-13 | Escherichia coli | PICU | Endotracheal Tube Secretion | Positive | Negative | Negative | Positive | 0.12 | 0.38 |
| 75 | Jun-13 | Escherichia coli | PICU | Urine | Positive | Negative | Negative | Positive | 0.12 | 0.38 |
| 76 | Jun-13 | Klebsiella pneumoniae ssp pneumoniae | SICU | Blood | Positive | Positive | Positive | Positive | 0.38 | 1.50 |
| 77 | Jul-13 | Klebsiella pneumoniae ssp pneumoniae | NICU | Blood | Positive | Positive | Negative | Negative | 0.38 | 0.38 |
| 78 | Jul-13 | Escherichia coli | NICU | Blood | Positive | Negative | Negative | Negative | 0.12 | 0.38 |
| 79 | Jul-13 | Klebsiella pneumoniae ssp pneumoniae | MICU | Sputum | Positive | Positive | Negative | Positive | 0.25 | 0.75 |
| 80 | Jul-13 | Klebsiella pneumoniae ssp pneumoniae | SICU | Endotracheal Tube Secretion | Positive | Positive | Positive | Positive | 0.19 | 0.38 |
| 81 | Jul-13 | Escherichia coli | PICU | Urine | Positive | Negative | Negative | Positive | 0.12 | 0.38 |
| 82 | Jul-13 | Escherichia coli | SICU | Urine | Positive | Negative | Positive | Negative | 0.25 | 0.50 |
| 83 | Jul-13 | Klebsiella pneumoniae ssp pneumoniae | MICU | BAL | Positive | Positive | Negative | Positive | 0.25 | 0.75 |
| 84 | Jul-13 | Escherichia coli | MICU | Urine | Positive | Negative | Negative | Negative | 0.09 | 0.25 |
| 85 | Jul-13 | Citrobacter amalonaticus | PICU | Urine | Positive | Positive | Negative | Negative | 0.12 | 0.19 |
| 86 | Jul-13 | Klebsiella pneumoniae ssp pneumoniae | MICU | Sputum | Positive | Positive | Positive | Positive | 0.25 | 0.50 |
| 87 | Jul-13 | Enterobacter cloacae | NICU | Eye Swab | Negative + AmpC | Negative | Negative | Negative | 0.09 | 0.25 |
| 88 | Jul-13 | Enterobacter cloacae | TICU | Blood | Negative + AmpC | Negative | Negative | Negative | 0.09 | 0.25 |
| 89 | Jul-13 | Enterobacter aerogenes | TICU | Blood | Positive | Positive | Negative | Negative | 0.09 | 0.38 |
| 90 | Aug-13 | Escherichia coli | MICU | Ascitic fluid | Positive | Negative | Positive | Positive | 0.12 | 0.25 |
| 91 | Aug-13 | Klebsiella pneumoniae ssp pneumoniae | MICU | Urine | Positive | Positive | Negative | Positive | 0.38 | 0.75 |
| 92 | Aug-13 | Enterobacter aerogenes | TICU | Sputum | Positive | Positive | Negative | Negative | 0.25 | 0.38 |
| 93 | Aug-13 | Klebsiella pneumoniae ssp pneumoniae | MICU | Sputum | Positive | Positive | Positive | Positive | 0.19 | 0.75 |
| 94 | Sep-13 | Escherichia coli | NICU | Blood | Positive | Negative | Negative | Positive | 0.19 | 0.75 |
| 95 | Sep-13 | Klebsiella pneumoniae ssp pneumoniae | PICU | Urine | Positive | Positive | Positive | Positive | 0.25 | 0.5 |
| 96 | Sep-13 | Escherichia coli | MICU | Urine | Positive | Negative | Positive | Positive | 0.09 | 0.38 |
| 97 | Sep-13 | Klebsiella pneumoniae ssp pneumoniae | MICU | Urine | Positive | Positive | Positive | Positive | 0.25 | 0.75 |
| 98 | Sep-13 | Escherichia coli | SICU | Sputum | Positive | Negative | Negative | Positive | 0.12 | 0.50 |
| 99 | Sep-13 | Klebsiella pneumoniae ssp pneumoniae | MICU | Sputum | Positive | Positive | Positive | Positive | 0.25 | 0.75 |
| 100 | Sep-13 | Klebsiella pneumoniae ssp pneumoniae | PICU | Blood | Positive | Negative | Positive | Positive | 0.12 | 0.25 |
| 101 | Sep-13 | Escherichia coli | PICU | Urine | Positive | Negative | Negative | Positive | 0.06 | 0.25 |
| 102 | Sep-13 | Klebsiella pneumoniae ssp pneumoniae | MICU | Sputum | Positive | Positive | Positive | Positive | 0.19 | 0.38 |
| 103 | Sep-13 | Klebsiella pneumoniae ssp pneumoniae | NICU | Central line Tip | Positive | Positive | Negative | Positive | 0.25 | 0.50 |
| 104 | Sep-13 | Escherichia coli | TICU | Sputum | Positive | Negative | Positive | Positive | 0.19 | 0.25 |
| 105 | Oct-13 | Escherichia coli | SICU | Blood | Positive | Negative | Negative | Positive | 0.06 | 0.19 |
| 106 | Oct-13 | Escherichia coli | SICU | Sputum | Positive | Positive | Positive | Negative | 0.06 | 0.19 |
| 107 | Oct-13 | Escherichia coli | SICU | Urine | Positive | Negative | Negative | Positive | 0.09 | 0.25 |
| 108 | Oct-13 | Klebsiella pneumoniae ssp pneumoniae | SICU | Blood | Positive | Positive | Positive | Positive | 0.38 | 1.00 |
| 109 | Oct-13 | Escherichia coli | TICU | Sputum | Positive | Positive | Positive | Negative | 0.06 | 0.25 |
ESBL: extended-spectrum -lactamase; MIC: minimum inhibitory concentration; White: susceptible; grey: non-susceptible, susceptibility was reported according to Clinical Laboratory Standards Institute (CLSI) breakpoints (Clinical Laboratory Standards Institute, 2020). m: month; y: year; MICU: Medical Intensive Care Unit; NICU: Neonatal Intensive Care Unit; PICU: Pediatric Intensive Care Unit; SICU: Surgical Intensive Care Unit; TICU: Trauma Intensive Care Unit; CZA: ceftazidime-avibactam; C/T: ceftolozane-tazobactam.