FIG. 5.

Effect of Fe protein redox state on reaction on DRAG-dependent reactivation. K. pneumoniae cultures were derepressed for nitrogen fixation and inactivated by addition of 0.5 mM ammonium acetate as in Fig. 4. The extent of modification of Fe protein was monitored by anti-Fe protein immunoblotting. Traces represent data from ⧫ UN5482 (draTGB, wild-type background) (⧫), UN5484 (nifD background) (◊), UN5485 (nifJ background) (■), and UN5486 (nifF background) (□). The data represent averages from four experiments. The standard deviation for each datum point is <30%. The beginning of reaction (inset) was defined as the last datum point at which modification was maximal for each experiment.