Table 2.
Average lengths and GC contents of ITS regions and free energy of 5.8S secondary structures of five species of Lycium.
| Primer pairs | Code | No. | Type | Length(bp)(SD) | GC content (%) (SD) | ΔG(kcal·mol-1) (SD) | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ITS | ITS1 | 5.8S | ITS2 | ITS1 | 5.8S | ITS2 | 5.8S | ITS2 | ||||
| ITS1/ITS4 | LD | 7 | P | 572 (35.4) | 197.6 (18.5) | 157.1 (3.0) | 217.1 (31.7) | 45.0 (0.0) | 45.3 (0.0) | 47.8 (0.0) | −45.0 (3.7) | −66.3 (9.4) |
| LTΔ | 12 | P | 571 (1.7) | 199.3 (3.8) | 153.5 (3.0) | 218.2 (0.8) | 44.3 (0.0) | 45.1 (0.0) | 49.2 (0.0) | −27.1 (0.9) | −66.8 (1.1) | |
| LRΔ | 1 | F | 687 (0.0) | 251 (0.0) | 165 (0.0) | 271 (0.0) | 62.2 (0.0) | 50.91 (0.0) | 63.5 (0.0) | −41.4 (0.0) | −110.7 (0.0) | |
| 5 | P | 588 (4.5) | 219.8 (2.1) | 155.6 (0.8) | 212.6 (3.2) | 59.5 (0.0) | 47.9 (0.0) | 58.9 (0.0) | −44.72 (5.0) | −80.7 (0.2) | ||
| LBVA | 1 | F | 691 (0.0) | 251 (0.0) | 159 (0.0) | 281 (0.0) | 62.2 (0.0) | 50.9 (0.0) | 64.4 (0.0) | −41.4 (0.0) | −122.2 (0.0) | |
| 9 | P | 583.7 (24.1) | 203 (23.7) | 157.0 (0.0) | 213.7 (0.5) | 51.7 (0.0) | 45.1 (0.0) | 50.9 (0.0) | −46.5 (1.0) | −72.6 (7.1) | ||
| LCΔ | 5 | F | 657 (25.5) | 231.2 (24.7) | 154.8 (2.64) | 268.2 (6.8) | 58.9 (0.0) | 54.0 (0.0) | 59.6 (0.0) | −46.06 (4.4) | −104.56 (9.2) | |
| 118 | P | 565.9 (16.4) | 199.4 (13.1) | 154.1 (3.7) | 212.6 (11.5) | 47.8 (0.1) | 46.3 (0.0) | 50.9 (0.0) | −47.8 (2.8) | −67.3 (8.2) | ||
| ALL | 7 | F | 666.1 (26.0) | 236.9 (22.7) | 156.9 (4.3) | 270.4 (7.2) | 59.8 (0.0) | 53.1 (0.0) | 60.8 (0.0) | −44.7 (4.3) | −108 (9.9) | |
| 151 | P | 567.3 (17.0) | 200.0 (14.0) | 154.4 (3.6) | 213.0 (12.0) | 47.9 (0.1) | 46.1 (0.0) | 50.8 (0.0) | −47.4 (2.9) | −67.7 (7.7) | ||
| P1/P2 | LC | 19 | F | 657.5 (36.7) | 229.6 (29.9) | 161.3 (6.9) | 266.6 (7.4) | 59.0 (0.0) | 50.9 (0.0) | 60.4 (0.0) | −45.0 (4.1) | −108.2 (4.5) |
| 3 | P | 684.7 (0.5) | 265.0 (2.2) | 158.0 (0.0) | 261.7 (1.9) | 43.4 (0.0) | 47.1 (0.0) | 52.6 (0.0) | −46.5 (0.8) | −89.5 (8.9) | ||
F, putative functional ITS sequences; P, presumed ITS pseudogene sequences; SD, standard deviation; Δ, Direct sequencing succeeded.