. 2022 Aug 25;13:950587. doi: 10.3389/fmicb.2022.950587

Table 6.

Effects of different contents of peNDF_>1.18 in diets on rumen microbial OTUs and alpha diversity of goats.

Items	Dietary peNDF _>1.18¹					SEM	p -value
	32.97%	29.93%	28.14%	26.48%	24.75%
16S rRNA gene sequence analysis
Effective sequences	30,923	31,948	30,293	30,977	33,370	743.48	0.779
OUT number	87.00^a	52.67^b	51.00^b	46.67^b	54.67^b	4.59	0.008
Chao1	126.89^a	64.20^ab	57.98^ab	53.95^b	65.50^ab	9.24	0.038
Shannon	1.30^a	0.61^c	0.56^c	0.60^c	0.82^b	0.07	< 0.001
Simpson	0.62^a	0.23^c	0.20^c	0.23^c	0.31^b	0.04	< 0.001
ITS sequence analysis
Effective sequences	32,721	33,025	33,115	33,258	34,944	827.65	0.948
OUT number	458^a	406^bc	381^c	441^b	354^c	80.59	< 0.001
Chao1	422.6^ab	404.0^b	357.2^bc	482.3^a	333.0^c	19.96	< 0.001
Shannon	3.910^a	3.051^b	3.165^b	3.113^b	2.644^c	0.077	< 0.001
Simpson	0.939^a	0.837^b	0.854^b	0.835^b	0.728^c	0.008	< 0.001

Different dietary peNDF_>1.18 (particle size >1.18 mm) contents of 32.97, 29.93, 28.14, 26.48, and 24.75% were obtained by chopping or crusher crushing the forage into the following lengths: long (7 cm), medium (4 cm), short (1 cm), fine (5 mm sieve), very fine (1 mm sieve).

Different letters^a−c denote p < 0.05.