. 2022 Aug 25;13:950587. doi: 10.3389/fmicb.2022.950587

Table 9.

Effects of dietary peNDF_>1.18 level on the functional prediction of rumen archaea (%).

Items	Dietary peNDF _>1.18¹					SEM	p -value
	32.97%	29.93%	28.14%	26.48%	24.75%
Metabolism
Amino acid metabolism	10.258^a	10.186^ab	10.178^b	10.184^ab	10.048^c	0.019	< 0.001
Biosynthesis of other secondary metabolites	1.066^b	1.074^b	1.097^a	1.069^b	1.003^c	0.009	< 0.001
Carbohydrate metabolism	10.109^d	10.234^ab	10.285^a	10.183^bc	10.163^c	0.017	< 0.001
Energy metabolism	6.009^a	6.002^a	6.034^a	6.059^a	5.828^b	0.023	< 0.001
Enzyme families	2.231^a	2.236^a	2.233^a	2.229^a	2.199^b	0.004	< 0.001
Glycan biosynthesis and metabolism	2.704^a	2.712^a	2.716^a	2.735^a	2.415^b	0.033	< 0.001
Lipid metabolism	2.745^b	2.702^c	2.740^b	2.738^b	2.829^a	0.012	< 0.001
Metabolism of cofactors and vitamins	4.619^a	4.604^a	4.543^b	4.583^ab	4.418^c	0.020	< 0.001
Metabolism of other amino acids	1.536^b	1.545^ab	1.563^a	1.543^ab	1.463^c	0.009	< 0.001
Metabolism of terpenoids and polyketides	1.746^a	1.755^a	1.723^bc	1.738^ab	1.702^c	0.005	< 0.001
Nucleotide metabolism	4.344^a	4.338^a	4.282^b	4.343^a	4.239^c	0.012	< 0.001
Xenobiotics biodegradation and metabolism	1.593^b	1.573^b	1.515^d	1.546^c	1.652^a	0.013	< 0.001
Cellular processes
Cell growth and death	0.562^b	0.574^a	0.558^b	0.565^b	0.542^c	0.003	< 0.001
Cell motility	2.554^b	2.485^b	2.728^a	2.541^b	2.860^a	0.039	< 0.001
Transport and catabolism	0.365^a	0.344^b	0.370^a	0.369^a	0.334^b	0.004	< 0.001
Environmental information processing
Membrane transport	9.374^b	9.550^b	9.511^b	9.374^b	10.242^a	0.091	< 0.001
Signal transduction	1.489^c	1.456^c	1.538^b	1.491^c	1.586^a	0.013	< 0.001
Signaling molecules and interaction	0.172^b	0.171^b	0.168^b	0.171^b	0.161^a	0.001	< 0.001
Genetic information processing
Folding, sorting and degradation	2.588^b	2.582^b	2.589^b	2.609^b	2.523^a	0.008	< 0.001
Replication and repair	9.607^a	9.588^a	9.502^b	9.646^a	9.455^b	0.020	< 0.001
Transcription	2.699^b	2.662^bc	2.631^c	2.644^bc	2.830^a	0.020	< 0.001
Translation	6.144^b	6.142^b	6.100^bc	6.209^a	6.085^c	0.012	<0.001
Human diseases
Cancers	0.106	0.107	0.107	0.106	0.106	0.001	0.179
Cardiovascular diseases	0.213	0.215	0.214	0.213	0.212	0.001	0.159
Infectious diseases	0.245^a	0.242^ab	0.240^bc	0.240^bc	0.238^c	0.001	0.001
Metabolic diseases	0.116^b	0.114^c	0.117^ab	0.119^a	0.115^bc	0.001	< 0.001
Neurodegenerative diseases	0.111^a	0.105^b	0.110^ab	0.110^ab	0.107^ab	0.001	0.028
Organismal systems
Environmental adaptation	0.151^c	0.151^c	0.160^a	0.154^b	0.160^a	0.001	< 0.001
Nervous system	0.107^a	0.104^bc	0.103^c	0.105^b	0.104^b	0.001	< 0.001
Unclassified

Different dietary peNDF_>1.18 (particle size >1.18 mm) contents of 32.97, 29.93, 28.14, 26.48, and 24.75% were obtained by chopping or crusher crushing the forage into the following lengths: long (7 cm), medium (4 cm), short (1 cm), fine (5 mm sieve), very fine (1 mm sieve).

Different letters^a−d denote p < 0.05.