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Western immunoblot analysis of polar flagellin production. Blots were reacted for 2 h with antiserum (1:1,000 dilution) directed against polar flagellins (Fla). The strain numbers are indicated above the lanes. The polar flagellins are similar in molecular size and comigrate in the resolving gel system used. An antiserum-reactive, nonflagellin band serves as a control for the amount of whole cells loaded in each lane.
